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Effects of acute normovolemic hemodilution on healing of gastric anastomosis in rats

Title
Effects of acute normovolemic hemodilution on healing of gastric anastomosis in rats
Author
정미애
Keywords
Hemodilution; Anastomotic leak; Experimental animal model
Issue Date
2018-12
Publisher
KOREAN SURGICAL SOCIETY
Citation
ANNALS OF SURGICAL TREATMENT AND RESEARCH, v. 95, no. 6, page. 312-318
Abstract
Purpose: Acute normovolemic hemodilution (ANH) is an autologous transfusion method, using blood collected during surgery, to reduce the need for allogeneic blood transfusion. ANH is controversial because it may lead to various complications. Among the possible complications, anastomotic leakage is one that would have a significant effect on the operation outcome. However, the relationship between ANH and anastomotic site healing requires additional research. Therefore, we conducted this prospective study of ANH, comparing it with standard intraoperative management, undergoing gastric anastomosis in rats.Methods: Sixteen Sprague-Dawley rats were randomly assigned to three groups: group A, surgery with ANH; group N, surgery with standard intraoperative management; and group C, sham surgery with standard intraoperative management. ANH was performed in group A animals by, removing 5.8-6.6 mL of blood and replacing it with 3 times as much crystalloid. All rats were enthanized on postoperative day 6, and histopathologic analyses were performed.Results: The mean hematocrit values, after hemodilution were 22.0% (range, 18.0%-29.0%), group A; 33.0% (29.0%-35.0%), group N; and 32.5% (29.0%-34.0%), group C. There were significant differences between groups A and N (P = 0.019, P = 0.009, P = 0.004, P = 0.039, and P = 0.027), and between groups N and C (P = 0.006, P = 0.027, P = 0.04, P = 0.008, and P = 0.009) with respect to inflammatory cell numbers, neovascularization, fibroblast numbers, edema and necrosis, respectively; there were no differences between groups A and N.Conclusion: In rat model, anastomotic complications did not increase in the ANH group, compared with the standard intraoperative management group.
URI
https://www.astr.or.kr/DOIx.php?id=10.4174/astr.2018.95.6.312http://repository.hanyang.ac.kr/handle/20.500.11754/120837
ISSN
2288-6575; 2288-6796
DOI
10.4174/astr.2018.95.6.312
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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