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Utilization of agricultural waste biomass by cellulolytic isolate Enterobacter sp. SUK-Bio

Title
Utilization of agricultural waste biomass by cellulolytic isolate Enterobacter sp. SUK-Bio
Author
전병훈
Keywords
Cellulolytic enzyme; Congo red; Dubos media; Enterobacter sp. SUK-Bio; Sorghum husk
Issue Date
2018-10
Publisher
Elsevier BV
Citation
Agriculture and Natural Resources, v. 52, no. 5, page. 399-406
Abstract
A cellulolytic bacterium was isolated from plant litter soil and identified as Enterobacter sp. SUK-Bio. This isolate was investigated for its utilization of different cellulosic materials (carboxymethyl cellulose, sugarcane trash, grass powder, sorghum husk, wheat straw and water hyacinth). Utilization of sorghum husk was comparatively more than for the other cellulosic materials used, producing higher cellulolytic and hemicellulolytic enzymes (filter paperase (0.15 U/mL), β-glucosidase (37.10 U/mL), endoglucanase (12.24 U/mL), exoglucanase (2.52 U/mL), xylanase (26.26 U/mL) and glucoamylase (33.26 U/mL)) on day 8 of incubation. Furthermore, it produced the maximum reducing sugar production (554 mg/L) at a rate of 3.84 mg/h/L. Fourier-transform infrared spectroscopy analysis of sorghum husk revealed functional groups changes and a decrease in the total crystallinity ratio after microbial degradation. The effects of supplementation of different metals additives, thermal stability and pH on cellulolytic enzymes were also studied.
URI
https://www.sciencedirect.com/science/article/pii/S2452316X17301187?via%3Dihubhttp://repository.hanyang.ac.kr/handle/20.500.11754/120438
ISSN
2452-316X
DOI
10.1016/j.anres.2018.10.019
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > EARTH RESOURCES AND ENVIRONMENTAL ENGINEERING(자원환경공학과) > Articles
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