Inducing angiogenesis with the controlled release of nitric oxide from biodegradable and biocompatible copolymeric nanoparticles
- Title
- Inducing angiogenesis with the controlled release of nitric oxide from biodegradable and biocompatible copolymeric nanoparticles
- Author
- 이동윤
- Keywords
- mPEG-PLGA nanoparticles; sprouting angiogenesis; low concentration of nitric oxide; liposomal nanoparticles; amphiphilic polymers
- Issue Date
- 2018-10
- Publisher
- DOVE MEDICAL PRESS LTD
- Citation
- INTERNATIONAL JOURNAL OF NANOMEDICINE, v. 13, page. 6517-6530
- Abstract
- Purpose: Nitric oxide (NO) can be clinically applied at low concentrations to regulate angiogenesis. However, studies using small molecule NO donors (N-diazeniumdiolate, S-nitrosothiol, etc) have yet to meet clinical requirements due to the short half-life and initial burst-release profile of NO donors. In this study, we report the feasibility of methoxy poly(ethylene glycol)-b-poly(lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (NPs) as NO-releasing polymers (NO-NPs) for inducing angiogenesis.Materials and methods: The mPEG-PLGA copolymers were synthesized by typical ring-opening polymerization of lactide, glycolide and mPEG as macroinitiators. Double emulsion methods were used to prepare mPEG-PLGA NPs incorporating hydrophilic NONOate (diethylenetriamine NONOate).Results: This liposomal NP encapsulates hydrophilic diethylenetriamine NONOate (70%+/- 4%) more effectively than other previously reported materials. The application of NO-NPs at different ratios resulted in varying NO-release profiles with no significant cytotoxicity in various cell types: normal cells (fibroblasts, human umbilical vein endothelial cells and epithelial cells) and cancer cells (C6, A549 and MCF-7). The angiogenic potential of NO-NPs was confirmed in vitro by tube formation and ex vivo through an aorta ring assay. Tubular formation increased 189.8% in NO-NP-treated groups compared with that in the control group. Rat aorta exhibited robust sprouting angiogenesis in response to NO-NPs, indicating that NO was produced by polymeric NPs in a sustained manner.Conclusion: These findings provide initial results for an angiogenesis-related drug development platform by a straightforward method with biocompatible polymers.
- URI
- https://www.dovepress.com/inducing-angiogenesis-with-the-controlled-release-of-nitric-oxide-from-peer-reviewed-article-IJNhttps://repository.hanyang.ac.kr/handle/20.500.11754/120289
- ISSN
- 1178-2013
- DOI
- 10.2147/IJN.S174989
- Appears in Collections:
- COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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