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Steroid-Based Amphiphiles for Membrane Protein Study: The Importance of Alkyl Spacers for Protein Stability

Title
Steroid-Based Amphiphiles for Membrane Protein Study: The Importance of Alkyl Spacers for Protein Stability
Author
채필석
Keywords
amphiphiles; membrane proteins; molecular design; protein stability; steroids
Issue Date
2018-07
Publisher
WILEY-V C H VERLAG GMBH
Citation
CHEMBIOCHEM, v. 19, no. 13, page. 1433-1443
Abstract
Membrane proteins allow effective communication between cells and organelles and their external environments. Maintaining membrane protein stability in a non-native environment is the major bottleneck to their structural study. Detergents are widely used to extract membrane proteins from the membrane and to keep the extracted protein in a stable state for downstream characterisation. In this study, three sets of steroid-based amphiphilesglyco-diosgenin analogues (GDNs) and steroid-based pentasaccharides either lacking a linker (SPSs) or containing a linker (SPS-Ls)have been developed as new chemical tools for membrane protein research. These detergents were tested with three membrane proteins in order to characterise their ability to extract membrane proteins from the membrane and to stabilise membrane proteins long-term. Some of the detergents, particularly the SPS-Ls, displayed favourable behaviour with the tested membrane proteins. This result indicates the potential utility of these detergents as chemical tools for membrane protein structural study and a critical role of the simple alkyl spacer in determining detergent efficacy.
URI
https://onlinelibrary.wiley.com/doi/abs/10.1002/cbic.201800106http://repository.hanyang.ac.kr/handle/20.500.11754/119427
ISSN
1439-4227; 1439-7633
DOI
10.1002/cbic.201800106
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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