Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이진원 | - |
dc.date.accessioned | 2019-12-08T14:51:22Z | - |
dc.date.available | 2019-12-08T14:51:22Z | - |
dc.date.issued | 2018-07 | - |
dc.identifier.citation | BIOCHEMICAL JOURNAL, v. 475, no. 13, page. 2127-2151 | en_US |
dc.identifier.issn | 0264-6021 | - |
dc.identifier.issn | 1470-8728 | - |
dc.identifier.uri | http://www.biochemj.org/content/475/13/2127 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/119419 | - |
dc.description.abstract | YlaD, a membrane-anchored anti-sigma (sigma) factor of Bacillus subtilis, contains a HX3CXXC motif that functions as a redox-sensing domain and belongs to one of the zinc (Zn)-co-ordinated anti-sigma factor families. Despite previously showing that the YlaC transcription is controlled by YlaD, experimental evidence of how the YlaC-YlaD interaction is affected by active cysteines and/or metal ions is lacking. Here, we showed that the P-yla promoter is autoregulated solely by YlaC. Moreover, reduced YlaD contained Zn and iron, while oxidized YlaD did not. Cysteine substitution in YlaD led to changes in its secondary structure; Cys3 had important structural functions in YlaD, and its mutation caused dissociation from YlaC, indicating the essential requirement of a HX3CXXC motif for regulating interactions of YlaC with YlaD. Analyses of the far-UV CD spectrum and metal content revealed that the addition of Mn ions to Zn-YlaD changed its secondary structure and that iron was substituted for manganese (Mn). The ylaC gene expression using beta Glu activity from P-yla:gusA was observed at the late-exponential and early-stationary phase, and the ylaC-overexpressing mutant constitutively expressed gene transcripts of clpP and sigH, an important alternative s factor regulated by ClpXP. Collectively, our data demonstrated that YlaD senses redox changes and elicits increase in Mn ion concentrations and that, in turn, YlaD-mediated transcriptional activity of YlaC regulates sporulation initiation under oxidative stress and Mn-substituted conditions by regulating clpP gene transcripts. This is the first report of the involvement of oxidative stress-responsive B. subtilis extracytoplasmic function s factors during sporulation via a Mn-dependent redox-sensing molecular switch. | en_US |
dc.description.sponsorship | This work was supported by the Research Fellowship of the BK21plus project. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | PORTLAND PRESS LTD | en_US |
dc.subject | EXTRACYTOPLASMIC FUNCTION FAMILY | en_US |
dc.subject | GENERAL STRESS-RESPONSE | en_US |
dc.subject | STREPTOMYCES-COELICOLOR | en_US |
dc.subject | ESCHERICHIA-COLI | en_US |
dc.subject | HYDROGEN-PEROXIDE | en_US |
dc.subject | OXIDATIVE STRESS | en_US |
dc.subject | SUPEROXIDE STRESS | en_US |
dc.subject | ZINC-BINDING | en_US |
dc.subject | FACTOR RSRA | en_US |
dc.subject | RESISTANCE | en_US |
dc.title | Anti-sigma factor YlaD regulates transcriptional activity of sigma factor YlaC and sporulation via manganese-dependent redox-sensing molecular switch in Bacillus subtilis | en_US |
dc.type | Article | en_US |
dc.relation.volume | 475 | - |
dc.identifier.doi | 10.1042/BCJ20170911 | - |
dc.relation.page | 2127-2151 | - |
dc.relation.journal | BIOCHEMICAL JOURNAL | - |
dc.contributor.googleauthor | Kwak, Min-Kyu | - |
dc.contributor.googleauthor | Ryu, Han-Bong | - |
dc.contributor.googleauthor | Song, Sung-Hyun | - |
dc.contributor.googleauthor | Lee, Jin-Won | - |
dc.contributor.googleauthor | Kang, Sa-Ouk | - |
dc.relation.code | 2018002886 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | jwl | - |
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