Identification and Functional Analysis of the psaD Promoter of Chlorella vulgaris Using Heterologous Model Strains
- Title
- Identification and Functional Analysis of the psaD Promoter of Chlorella vulgaris Using Heterologous Model Strains
- Author
- 진언선
- Keywords
- Chlorella vulgaris; native promoter; photosystemI protein D (psaD); heterologous expression
- Issue Date
- 2018-07
- Publisher
- MDPI
- Citation
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 19, no. 7, Article no. 1969
- Abstract
- Chlorella has great potential as a bio-factory for production of value-added compounds. To produce the desired chemicals more efficiently in Chlorella, genetic tools for modification of Chlorella need to be developed, especially an endogenous promoter. In this study, the promoter of photosystem I protein D (psaD) from Chlorella vulgaris UTEX395 was identified. Computational analysis revealed the presence of several putative cis-acting elements, including a potential core element, and light-responsive or stress-responsive elements. Gene expression analysis in heterologous expression system in (C)hlamydomonas reinhardtii and Nicotiana benthamiana showed that CvpsaD promoter can be used to drive the expression of genes. Functional analysis of this promoter suggested that the initiator element (Inr) is important for its function (i.e., TATA-less promoter) and that an additional factor (e.g., downstream of the transcriptional start site) might be needed for light response. We have shown that the CvpsaD promoter is functional, but not sufficiently strong, both in microalgae and higher plant.
- URI
- https://www.mdpi.com/1422-0067/19/7/1969https://repository.hanyang.ac.kr/handle/20.500.11754/119411
- ISSN
- 1422-0067
- DOI
- 10.3390/ijms19071969
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
- Files in This Item:
- Identification and Functional Analysis of the psaD Promoter of Chlorella vulgaris Using Heterologous Model Strains.pdfDownload
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