Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 최준호 | - |
dc.date.accessioned | 2019-12-08T13:35:05Z | - |
dc.date.available | 2019-12-08T13:35:05Z | - |
dc.date.issued | 2018-07 | - |
dc.identifier.citation | MOLECULAR CELL, v. 71, no. 2, page. 244-255.e5 | en_US |
dc.identifier.issn | 1097-2765 | - |
dc.identifier.issn | 1097-4164 | - |
dc.identifier.uri | https://www.cell.com/molecular-cell/fulltext/S1097-2765(18)30443-X?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS109727651830443X%3Fshowall%3Dtrue | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/119331 | - |
dc.description.abstract | tRNAs are subject to numerous modifications, including methylation. Mutations in the human N-7-methylguanosine (m(7)G) methyltransferase complex METTL1/WDR4 cause primordial dwarfism and brain malformation, yet the molecular and cellular function in mammals is not well understood. We developed m(7)G methylated tRNA immunoprecipitation sequencing (MeRIP-seq) and tRNA reduction and cleavage sequencing (TRAC-seq) to reveal the m(7)G tRNA methylome in mouse embryonic stem cells (mESCs).A subset of 22 tRNAs is modified at a ''RAGGU'' motif within the variable loop. We observe increased ribosome occupancy at the corresponding codons in Mettl1 knockout mESCs, implying widespread effects on tRNA function, ribosome pausing, and mRNA translation. Translation of cell cycle genes and those associated with brain abnormalities is particularly affected. Mettl1 or Wdr4 knockout mESCs display defective self-renewal and neural differentiation. Our study uncovers the complexity of the mammalian m(7)G tRNA methylome and highlights its essential role in ESCs with links to human disease. | en_US |
dc.description.sponsorship | We thank Ronald Mathieu (Boston Children's Hospital) for help with flow cytometry analysis. S.L. was supported by a Damon Runyon-Sohn Pediatric Fellowship from Damon Runyon Cancer Research Foundation (DRSG-7-13) and a Young Investigator grant from Alex's Lemonade Stand Foundation (GR-000000296). R.I.G. was supported by grants from the US National Institute of General Medical Sciences (NIGMS) (R01GM086386) and National Cancer Institute (R01CA211328). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | CELL PRESS | en_US |
dc.subject | YEAST TRANSFER-RNA | en_US |
dc.subject | GENE | en_US |
dc.subject | METHYLATION | en_US |
dc.subject | REVEALS | en_US |
dc.subject | COMPLEX | en_US |
dc.subject | WDR4 | en_US |
dc.subject | 7-METHYLGUANOSINE | en_US |
dc.subject | PHENOTYPE | en_US |
dc.subject | FORM | en_US |
dc.subject | CODE | en_US |
dc.title | Mettl1/Wdr4-Mediated m(7)G tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation | en_US |
dc.type | Article | en_US |
dc.relation.no | 2 | - |
dc.relation.volume | 71 | - |
dc.identifier.doi | 10.1016/j.molcel.2018.06.001 | - |
dc.relation.page | 244-244 | - |
dc.relation.journal | MOLECULAR CELL | - |
dc.contributor.googleauthor | Lin, Shuibin | - |
dc.contributor.googleauthor | Liu, Qi | - |
dc.contributor.googleauthor | Lelyveld, Victor S. | - |
dc.contributor.googleauthor | Choe, Junho | - |
dc.contributor.googleauthor | Szostak, Jack W. | - |
dc.contributor.googleauthor | Gregory, Richard I. | - |
dc.relation.code | 2018000040 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | jcho2711 | - |
dc.identifier.researcherID | E-3410-2019 | - |
dc.identifier.orcid | http://orcid.org/0000-0002-2365-3755 | - |
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