Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 이근용 | - |
dc.date.accessioned | 2019-12-08T09:02:14Z | - |
dc.date.available | 2019-12-08T09:02:14Z | - |
dc.date.issued | 2018-06 | - |
dc.identifier.citation | CARBOHYDRATE POLYMERS, v. 197, page. 422-430 | en_US |
dc.identifier.issn | 0144-8617 | - |
dc.identifier.issn | 1879-1344 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0144861718306702?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/119053 | - |
dc.description.abstract | Hyaluronate-based hydrogels have been widely exploited as synthetic extracellular matrices in many tissue engineering applications, including cartilage tissue engineering. Hyaluronate-based hydrogels are typically prepared by chemical cross-linking reactions, in which chemical reagents may induce side effects, unless they are completely removed after the cross-linking reaction. We thus suggest the utilization of hybrid materials composed of hyaluronate as a main chain and alginate for physical cross-linking to simply form hydrogels in the presence of calcium ions under physiological conditions. In this study, we hypothesized that the introduction of biomimetic peptides to hyaluronate-alginate hybrid (HAH) hydrogels could be useful to regulate the chondrocyte phenotype, including chondrogenic differentiation. HAH was modified with the arginine-glycine-aspartate (RGD) peptide as a cell-matrix interaction motif and/or histidine-alanine-valine (HAV) as a cell-cell interaction motif. The HAV peptide is known to bind to cadherin, which is a key factor involved in homophilic cell-cell interactions as well as chondrogenesis. The viability and growth of mouse chondrocytes (ATDC5 cells) increased significantly when cultured on RGD-modified HAH hydrogels. Cell aggregates formed on HAV-modified HAH hydrogels, resulting in enhanced chondrogenic differentiation via enhanced cell-cell interactions by HAV modification. Interestingly, a synergistic effect of HAV and RGD peptides within HAH hydrogels on chondrogenesis was found in 3-D experiments in vitro. This approach to utilizing physically cross-linkable hyaluronate-based hydrogels presenting biomimetic peptides has potential applications in tissue engineering, including cartilage regeneration. | en_US |
dc.description.sponsorship | This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Ministry of Science and ICT (NRF-2016R1A2A2A10005086). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ELSEVIER SCI LTD | en_US |
dc.subject | Hyaluronate | en_US |
dc.subject | Alginate | en_US |
dc.subject | Arginine-glycine-aspartate | en_US |
dc.subject | Histidine-alanine-valine | en_US |
dc.subject | Chondrocyte phenotype | en_US |
dc.title | Hyaluronate-alginate hybrid hydrogels modified with biomimetic peptides for controlling the chondrocyte phenotype | en_US |
dc.type | Article | en_US |
dc.relation.volume | 197 | - |
dc.identifier.doi | 10.1016/j.carbpol.2018.06.016 | - |
dc.relation.page | 422-430 | - |
dc.relation.journal | CARBOHYDRATE POLYMERS | - |
dc.contributor.googleauthor | An, Hyoseok | - |
dc.contributor.googleauthor | Lee, Jae Won | - |
dc.contributor.googleauthor | Lee, Hyun Ji | - |
dc.contributor.googleauthor | Seo, Yerang | - |
dc.contributor.googleauthor | Park, Honghyun | - |
dc.contributor.googleauthor | Lee, Kuen Yong | - |
dc.relation.code | 2018002000 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | leeky | - |
dc.identifier.orcid | https://orcid.org/0000-0002-5759-5952 | - |
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