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Cytotoxicity measurement of Bisphenol A (BPA) and its substitutes using human keratinocytes

Title
Cytotoxicity measurement of Bisphenol A (BPA) and its substitutes using human keratinocytes
Author
계명찬
Keywords
Apoptosis; Bisphenol A; BPA substitutes; Endocrine disruptor; HaCaT
Issue Date
2018-06
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation
ENVIRONMENTAL RESEARCH, v. 164, page. 655-659
Abstract
Bisphenol-A (BPA) was first synthesized in the 1890s and has been used in many plastic products. However, BPA is known to act as an endocrine disruptor and has been found to be toxic to human health. Many alternative substances have been developed to replace BPA, but it is still widely used worldwide. In this study, we identified the potential cytotoxicity of BPA by evaluating toxicity using human keratinocytes. Also, we evaluated cytotoxicity of BPA substitutes to determine their suitability as an alternative to BPA. The proliferation assay using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, flow cytometry and western blot analysis showed that BPA significantly affect cell viability, induction of apoptotic fraction and increased activation of DNA-damage marker protein. In addition, through the same experiments, the substitutes of BPA were shown to be significantly less toxic than BPA, and the least toxicity was observed with 1,4-cyclohexanedimethanol (CHDM) and terephthalic acid (TPA). In conclusion, this study suggests that cytotoxicity of BPA induces apoptosis of human keratinocytes, and that CHDM and TPA are the most suitable substitutes for BPA.
URI
https://www.sciencedirect.com/science/article/pii/S0013935118301750?via%3Dihubhttp://repository.hanyang.ac.kr/handle/20.500.11754/118873
ISSN
0013-9351; 1096-0953
DOI
10.1016/j.envres.2018.03.043
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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