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dc.contributor.author이민형-
dc.date.accessioned2019-12-08T02:45:02Z-
dc.date.available2019-12-08T02:45:02Z-
dc.date.issued2018-05-
dc.identifier.citationNANOSCALE, v. 10, no. 18, page. 8503-8514en_US
dc.identifier.issn2040-3364-
dc.identifier.issn2040-3372-
dc.identifier.urihttps://pubs.rsc.org/en/content/articlelanding/2018/NR/C8NR00427G#!divAbstract-
dc.identifier.urihttp://repository.hanyang.ac.kr/handle/20.500.11754/118674-
dc.description.abstractAcute lung injury (ALI) is a lung inflammatory disease for which pulmonary delivery of drug and gene could be a useful strategy. In this study, cholesterol-conjugated polyamidoamine (PAM-Chol) was synthesized and characterized as a carrier for combined delivery of anti-inflammatory gene and drug into the lungs by inhalation. The PAM-Chol formed self-assembled micelles in an aqueous solution with a critical micelle concentration of 0.22 mg ml(-1). An in vitro transfection assay to L2 lung epithelial cells showed that the PAM-Chol micelle had higher transfection efficiency than lipofectamine and polyethylenimine (25 kDa, PEI25k). As the anti-inflammatory drug, resveratrol was loaded into the cores of the PAM-Chol micelles using the oil-in-water emulsion/solvent evaporation method. In lipopolysaccharide (LPS)-activated macrophage cells, resveratrol-loaded PAM-Chol (PAM-Chol/Res) reduced pro-inflammatory cytokines, confirming the anti-inflammatory effects of resveratrol. In in vitro transfection assays to L2 cells, the PAM-Chol/Res micelles had transfection efficiency similar to that of PAM-Chol. The delivery of resveratrol or the heme oxygenase-1 gene (pHO-1) by inhalation was evaluated in an ALI animal model. Resveratrol delivery using the PAM-Chol/Res micelles inhibited the nuclear translocation of nuclear factor-kappa B (NF-kappa B) and reduced pro-inflammatory cytokines in the lungs. pHO-1 delivery using PAM-Chol induced HO-1 expression and reduced pro-inflammatory cytokines. However, the highest anti-inflammatory effects were obtained with combined delivery of pHO-1 and resveratrol using the pHO-1/PAM-Chol/Res complex, as demonstrated in cytokine assays and immunohistochemical studies. Therefore, the PAM-Chol micelle is an efficient carrier of resveratrol and pHO-1 into the lungs and could be useful for the treatment of ALI by inhalation.en_US
dc.description.sponsorshipThis work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2016R1D1A1B03934778) and the Ministry of Science and ICT (NRF-2017R1A2B4009036). It was also supported by the Bio & Medical Technology Development Program through NRF funded by the Ministry of Science and ICT (NRF-2016M3A9B4918833).en_US
dc.language.isoen_USen_US
dc.publisherROYAL SOC CHEMISTRYen_US
dc.subjectRESPIRATORY-DISTRESS-SYNDROMEen_US
dc.subjectHEME OXYGENASE-1 GENEen_US
dc.subjectPOLYAMIDOAMINE DENDRIMERSen_US
dc.subjectINFLAMMATORY MEDIATORSen_US
dc.subjectISCHEMIC BRAINen_US
dc.subjectPLASMID DNAen_US
dc.subjectINJURYen_US
dc.subjectRESVERATROLen_US
dc.subjectDEXAMETHASONEen_US
dc.subjectTHERAPYen_US
dc.titleSelf-assembled polymeric micelles for combined delivery of anti-inflammatory gene and drug to the lungs by inhalationen_US
dc.typeArticleen_US
dc.relation.no18-
dc.relation.volume10-
dc.identifier.doi10.1039/c8nr00427g-
dc.relation.page8503-8514-
dc.relation.journalNANOSCALE-
dc.contributor.googleauthorKim, Gyeungyun-
dc.contributor.googleauthorPiao, Chunxian-
dc.contributor.googleauthorOh, Jungju-
dc.contributor.googleauthorLee, Minhyung-
dc.relation.code2018000106-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF ENGINEERING[S]-
dc.sector.departmentDEPARTMENT OF BIOENGINEERING-
dc.identifier.pidminhyung-
dc.identifier.orcidhttps://orcid.org/0000-0002-7083-9296-
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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