Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 신흥수 | - |
dc.date.accessioned | 2019-12-07T20:43:14Z | - |
dc.date.available | 2019-12-07T20:43:14Z | - |
dc.date.issued | 2018-04 | - |
dc.identifier.citation | BIOMATERIALS, v. 165, page. 79-93 | en_US |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.issn | 1878-5905 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0142961218301480?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/118448 | - |
dc.description.abstract | Tendon-bone interface tissue is extremely challenging to engineer because it exhibits complex gradients of structure, composition, biologics, and cellular phenotypes. As a step toward engineering these transitional zones, we initially analyzed how different (topographical or biological) cues affect tenogenic differentiation of adipose-derived stem cells (ADSCs). We immobilized platelet-derived growth factor BB (PDGF-BB) using polydopamine (PD) chemistry on random and aligned nanofibers and investigated ADSC proliferation and tenogenic differentiation. Immobilized PDGF greatly enhanced the proliferation and tenogenic differentiation of ADSCs; however, nanofiber alignment had no effect. Interestingly, the PDGF immobilized aligned nanofiber group showed a synergistic effect with maximum expression of tenogenic markers for 14 days. We also generated a nanofiber surface with spatially controlled presentation of immobilized PDGF on an aligned architecture, mimicking native tendon tissue. A gradient of immobilized PDGF was able to control the phenotypic differentiation of ADSCs into tenocytes in a spatially controlled manner, as confirmed by analysis of the expression of tenogenic markers and immunofluorescence staining. We further explored the gradient formation strategy by generation of a symmetrical gradient on the nanofiber surface for the generation of a structure mimicking bone-patellar tendon -bone with provision for gradient immobilization of PDGF and controlled mineralization. Our study reveals that, together with biochemical cues, favorable topographical cues are important for tenogenic differentiation of ADSCs, and gradient presentation of PDGF can be used as a tool for engineering stem cell-based bone-tendon interface tissues. | en_US |
dc.description.sponsorship | This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2016R1A2B3009936). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ELSEVIER SCI LTD | en_US |
dc.subject | Bone-tendon interface | en_US |
dc.subject | Platelet-derived growth factor | en_US |
dc.subject | Gradient surfaces | en_US |
dc.subject | Tenogenic differentiation | en_US |
dc.title | Harnessing biochemical and structural cues for tenogenic differentiation of adipose derived stem cells (ADSCs) and development of an in vitro tissue interface mimicking tendon-bone insertion graft | en_US |
dc.type | Article | en_US |
dc.relation.volume | 165 | - |
dc.identifier.doi | 10.1016/j.biomaterials.2018.02.046 | - |
dc.relation.page | 79-93 | - |
dc.relation.journal | BIOMATERIALS | - |
dc.contributor.googleauthor | Perikamana, Sajeesh Kumar Madhurakkat | - |
dc.contributor.googleauthor | Lee, Jinkyu | - |
dc.contributor.googleauthor | Ahmad, Taufiq | - |
dc.contributor.googleauthor | Kim, Eun Mi | - |
dc.contributor.googleauthor | Byun, Hayeon | - |
dc.contributor.googleauthor | Lee, Sangmin | - |
dc.contributor.googleauthor | Shin, Heungsoo | - |
dc.relation.code | 2018001589 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | hshin | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.