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Vitamin E-based glycoside amphiphiles for membrane protein structural studies

Title
Vitamin E-based glycoside amphiphiles for membrane protein structural studies
Author
채필석
Keywords
CRYSTAL-STRUCTURE; BETA(2)-ADRENERGIC RECEPTOR; ALLOSTERIC MODULATION; FACIAL AMPHIPHILES; ELECTRON-TRANSFER; CRYSTALLIZATION; STABILIZATION; SOLUBILIZATION; FLEXIBILITY; DETERGENTS
Issue Date
2018-04
Publisher
ROYAL SOC CHEMISTRY
Citation
ORGANIC & BIOMOLECULAR CHEMISTRY, v. 16, no. 14, page. 2489-2498
Abstract
Membrane proteins play critical roles in a variety of cellular processes. For a detailed molecular level understanding of their biological functions and roles in disease, it is necessary to extract them from the native membranes. While the amphipathic nature of these bio-macromolecules presents technical challenges, amphiphilic assistants such as detergents serve as useful tools for membrane protein structural and functional studies. Conventional detergents are limited in their ability to maintain the structural integrity of membrane proteins and thus it is essential to develop novel agents with enhanced properties. Here, we designed and characterized a novel class of amphiphiles with vitamin E (i.e., -tocopherol) as the hydrophobic tail group and saccharide units as the hydrophilic head group. Designated vitamin E-based glycosides (VEGs), these agents were evaluated for their ability to solubilize and stabilize a set of membrane proteins. VEG representatives not only conferred markedly enhanced stability to a diverse range of membrane proteins compared to conventional detergents, but VEG-3 also showed notable efficacy toward stabilization and visualization of a membrane protein complex. In addition to hydrophile-lipophile balance (HLB) of detergent molecules, the chain length and molecular geometry of the detergent hydrophobic group seem key factors in determining detergent efficacy for membrane protein (complex) stability.
URI
https://pubs.rsc.org/en/content/articlelanding/2018/OB/C8OB00270C#!divAbstracthttp://repository.hanyang.ac.kr/handle/20.500.11754/118266
ISSN
1477-0520; 1477-0539
DOI
10.1039/c8ob00270c
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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