Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 주재범 | - |
dc.date.accessioned | 2019-12-07T11:10:51Z | - |
dc.date.available | 2019-12-07T11:10:51Z | - |
dc.date.issued | 2018-03 | - |
dc.identifier.citation | ACS APPLIED MATERIALS & INTERFACES, v. 10, no. 8, page. 6831-6840 | en_US |
dc.identifier.issn | 1944-8244 | - |
dc.identifier.uri | https://pubs.acs.org/doi/10.1021/acsami.7b15085 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/118015 | - |
dc.description.abstract | We utilized a fast Raman spectral mapping technique for fast detection of bacterial pathogens. Three-dimensional (3D) plasmonic nanopillar arrays were fabricated using the nanolithography-free process consisting of maskless Ar plasma treatment of a polyethylene terephthalate substrate and subsequent metal deposition. Bacterial pathogens were immobilized on the positively charged poly(L-lysine)-coated 3D plasmonic substrate through electrostatic interactions. Then, the bacterial surfaces were selectively labeled with antibody-conjugated surface-enhanced Raman scattering (SERS) nanotags, and Raman mapping images were collected and statistically analyzed for quantitative analysis of bacteria. Salmonella typhimurium was selected as a model pathogen bacterium to confirm the efficacy of our SERS imaging technique. Minimum number of Raman mapping points with statistical reliability was determined to reduce assay time. It was possible to get a statistically reliable standard calibration curve for 529 pixels (laser spot with 60 mu m interval), which required a total mapping time of 45 min to get a standard calibration curve for five different concentrations of bacteria in the 0 to 10(6) CFU/mL range. No amplification step was necessary for quantification because low-abundance target bacteria could be measured using the Raman spectral mapping technique. Therefore, this approach allows accurate quantification of bacterial pathogens without any culturing or enrichment process. | en_US |
dc.description.sponsorship | The National Research Foundation of Korea supported this work through grant numbers R11-2008-0061852 and K20904000004-12A0500-00410. This work was supported from the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through the Advanced Production Technology Development Program funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA-316080-04). This work was also supported by the Fundamental Research Program (PNK 5060) of the Korean Institute of Materials Science (KIMS) and the Agency for Defense Development through Chemical & Biological Detection Research Center (CBDRC). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | AMER CHEMICAL SOC | en_US |
dc.subject | surface-enhanced Raman scattering (SERS) | en_US |
dc.subject | culture-free detection | en_US |
dc.subject | nanopillar pattern | en_US |
dc.subject | bacterial pathogen | en_US |
dc.subject | SERS nanotag | en_US |
dc.title | Culture-Free Detection of Bacterial Pathogens on Plasmonic Nanopillar Arrays Using Rapid Raman Mapping | en_US |
dc.type | Article | en_US |
dc.relation.no | 8 | - |
dc.relation.volume | 10 | - |
dc.identifier.doi | 10.1021/acsami.7b15085 | - |
dc.relation.page | 6831-6840 | - |
dc.relation.journal | ACS APPLIED MATERIALS & INTERFACES | - |
dc.contributor.googleauthor | Ko, Juhui | - |
dc.contributor.googleauthor | Park, Sung-Gyu | - |
dc.contributor.googleauthor | Lee, Sangyeov | - |
dc.contributor.googleauthor | Wang, Xiaokun | - |
dc.contributor.googleauthor | Mun, Chaewon | - |
dc.contributor.googleauthor | Kim, Sunho | - |
dc.contributor.googleauthor | Kim, Dong-Ho | - |
dc.contributor.googleauthor | Choo, Jaebum | - |
dc.relation.code | 2018001712 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | jbchoo | - |
dc.identifier.orcid | http://orcid.org/0000-0003-3864-6459 | - |
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