Cpf1-Database: web-based genome-wide guide RNA library design for gene knockout screens using CRISPR-Cpf1
- Title
- Cpf1-Database: web-based genome-wide guide RNA library design for gene knockout screens using CRISPR-Cpf1
- Author
- 배상수
- Keywords
- HUMAN-CELLS; FUNCTIONAL GENOMICS; TARGET SITES; CPF1; SPECIFICITIES; ENDONUCLEASES; NUCLEASES; SYSTEM
- Issue Date
- 2018-03
- Publisher
- OXFORD UNIV PRESS
- Citation
- BIOINFORMATICS, v. 34, no. 6, page. 1077-1079
- Abstract
- Following the type II CRISPR-Cas9 system, type V CRISPR-Cpf1 endonucleases have been found to be applicable for genome editing in various organisms in vivo. However, there are as yet no web-based tools capable of optimally selecting guide RNAs (gRNAs) among all possible genome-wide target sites. Here, we present Cpf1-Database, a genome-wide gRNA library design tool for LbCpf1 and AsCpf1, which have DNA recognition sequences of 5'-TTTN-3' at the 5' ends of target sites. Cpf1-Database provides a sophisticated but simple way to design gRNAs for AsCpf1 nucleases on the genome scale. One can easily access the data using a straightforward web interface, and using the powerful collections feature one can easily design gRNAs for thousands of genes in short time.
- URI
- https://academic.oup.com/bioinformatics/article/34/6/1077/4665414https://repository.hanyang.ac.kr/handle/20.500.11754/117917
- ISSN
- 1367-4803; 1460-2059
- DOI
- 10.1093/bioinformatics/btx695
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > CHEMISTRY(화학과) > Articles
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