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MerTK mediates STAT3–KRAS/SRC-signaling axis for glioma stem cell maintenance

Title
MerTK mediates STAT3–KRAS/SRC-signaling axis for glioma stem cell maintenance
Author
KAUSHIKNEHA
Keywords
MerTK; self-renewal; KRAS; STAT3; SRC
Issue Date
2018-03
Publisher
TAYLOR & FRANCIS LTD
Citation
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, v. 46, page. 87-95, Supplement:2
Abstract
Receptor tyrosine kinase Mer (MerTK) has been shown to be highly expressed in Glioblastoma multiforme (GBM) in comparison to its healthy counterpart and is implicated in brain tumorigenesis. Clarifying the underlying mechanism of MerTK induced invasiveness would result in novel strategies to improve patient's response to chemotherapeutics. In vitro and in vivo assays were performed to examine the functional role of cancer stem sell (CSC) maintenance in MerTK associated invasiveness. In this article, we demonstrate that apart from GBM cells, MerTK is also upregulated in GBM stem-like cells and associated with an increased infiltrative potential of brain tumors in vivo. Silencing of MerTK suppressed the self-renewal of patient-derived GBM stem-like cells. The signaling mechanisms by which MerTK contributes to CSC maintenance have largely been obscure. Molecular analyses revealed that high expression of the signal transducer and activator of transcription 3 (STAT3)- Kirsten rat sarcoma viral oncogene homolog (KRAS) and proto-oncogene tyrosine-protein kinase SRC axis supports MerTK-induced CSC maintenance in GBM spheroids. Furthermore, a short-hairpin RNA-mediated MerTK knockdown effectively blocked invasiveness and N-cadherin expression in mouse xenografts. Collectively, our results uncover a critical function of MerTK in CSC maintenance. Considering the low basal level of MerTK expression in healthy brain cells, evaluation of MerTK as a therapeutic target should advance the research into better therapeutics for GBM.
URI
https://www.tandfonline.com/doi/full/10.1080/21691401.2018.1452022http://repository.hanyang.ac.kr/handle/20.500.11754/117891
ISSN
2169-1401; 2169-141X
DOI
10.1080/21691401.2018.1452022
Appears in Collections:
RESEARCH INSTITUTE[S](부설연구소) > THE RESEARCH INSTITUTE FOR NATURAL SCIENCES(자연과학연구소) > Articles
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