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dc.contributor.author계명찬-
dc.date.accessioned2019-12-05T18:03:35Z-
dc.date.available2019-12-05T18:03:35Z-
dc.date.issued2018-03-
dc.identifier.citationSENSORS, v. 18, no. 3, Article no. 875en_US
dc.identifier.issn1424-8220-
dc.identifier.urihttps://www.mdpi.com/1424-8220/18/3/875-
dc.identifier.urihttp://repository.hanyang.ac.kr/handle/20.500.11754/117712-
dc.description.abstractWe report bioluminescence analysis of matrix metalloproteinase (MMP) activity in biological substances using a surface-bound luciferase probe. Intein-fused luciferase protein enables site-specific biotinylation of luciferase in the presence of N-terminus cysteine-biotin via intein-mediated splicing process, resulting in a strong association with high bioluminescence signal onto a NeutrAvidin-coated surface. When the peptide substrate for MMP-7 was inserted into a region between luciferase and intein, the biotinylated probe detected MMP-7 activity by cleaving the peptide, and surface-induced bioluminescence signal was strongly reduced in the MMP-secreted media or mouse tissue extracts, compared with that in MMP-deficient control set. Our approach is anticipated to be useful for generating biotinylated proteins and for their applications in diagnosing MMP activity in human diseases.en_US
dc.description.sponsorshipThis study was supported by a grant of the Korea Healthcare Technology R&D Project, Ministry for Health, Welfare & Family Affairs, Korea (HI14C0106). The plasmid, pBAD-RLuc8-GyrA, was kindly provided by Professor Jianghong Rao at Stanford University (School of Medicine, Stanford, CA, USA).en_US
dc.language.isoen_USen_US
dc.publisherMDPIen_US
dc.subjectluciferaseen_US
dc.subjectbioluminescenceen_US
dc.subjectmatrix metalloproteinaseen_US
dc.subjectinteinen_US
dc.subjectbiotinylationen_US
dc.titleDetection of Matrix Metalloproteinase Activity by Bioluminescence via Intein-Mediated Biotinylation of Luciferaseen_US
dc.typeArticleen_US
dc.relation.no3-
dc.relation.volume18-
dc.identifier.doi10.3390/s18030875-
dc.relation.page1-10-
dc.relation.journalSENSORS-
dc.contributor.googleauthorNguyen, Duc Long-
dc.contributor.googleauthorKim, Hanim-
dc.contributor.googleauthorKim, Dasom-
dc.contributor.googleauthorLee, Jin Oh-
dc.contributor.googleauthorGye, Myung Chan-
dc.contributor.googleauthorKim, Young-Pil-
dc.relation.code2018007781-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF NATURAL SCIENCES[S]-
dc.sector.departmentDEPARTMENT OF LIFE SCIENCE-
dc.identifier.pidmcgye-


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