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Determination of glycated albumin using boronic acid-derived agarose beads on paper-based devices

Title
Determination of glycated albumin using boronic acid-derived agarose beads on paper-based devices
Author
성기훈
Keywords
DIABETES-MELLITUS; GLYCEMIC CONTROL; MICROFLUIDICS; HEMOGLOBIN; GLUCOSE; VALUES
Issue Date
2018-02
Publisher
AMER INST PHYSICS
Citation
BIOMICROFLUIDICS, v. 12, no. 1, Article no. 014111
Abstract
Self-monitoring of glycated albumin (GA), a useful glycemic marker, is an established method for preventing diabetes complications. Here, the paper-based lateral flow assay devices were developed for the sensitive detection of GA and the total human serum albumin (tHSA) in self-monitoring diabetes patients. Boronic acid-derived agarose beads were packed into a hole on a lateral flow channel. These well-coordinated agarose beads were used to capture GA through specific cis-diol interactions and to enhance the colorimetric signals by concentrating the target molecules. The devices exhibited large dynamic ranges (from 10 mu g/ml to 10 mg/ml for GA and from 10 mg/ml to 50 mg/ml for tHSA) and low detection limits (7.1 mu g/ml for GA and 4.7 mg/ml for tHSA), which cover the range of GA concentration in healthy plasma, which is 0.21-1.65 mg/ml (0.6%-3%). In determining the unknown GA concentrations in two commercial human plasma samples, the relative percentage difference between the values found by a standard ELISA kit and those found by our developed devices was 2.62% and 8.80%, which are within an acceptable range. The measurements of GA and tHSA were completed within 20 min for the total sample-to-answer diagnosis, fulfilling the demand for rapid analysis. Furthermore, the recovery values ranged from 99.4% to 110% in device accuracy tests. These results indicate that the developed paper-based device with boronic acid-derived agarose beads is a promising platform for GA and tHSA detection as applied to self-monitoring systems. Published by AIP Publishing.
URI
https://aip.scitation.org/doi/10.1063/1.5021395http://repository.hanyang.ac.kr/handle/20.500.11754/117627
ISSN
1932-1058
DOI
10.1063/1.5021395
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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