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dc.contributor.author계명찬-
dc.date.accessioned2019-12-05T15:32:26Z-
dc.date.available2019-12-05T15:32:26Z-
dc.date.issued2018-02-
dc.identifier.citationENVIRONMENTAL POLLUTION, v. 233, page. 833-843en_US
dc.identifier.issn0269-7491-
dc.identifier.issn1873-6424-
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0269749117321255?via%3Dihub-
dc.identifier.urihttp://repository.hanyang.ac.kr/handle/20.500.11754/117608-
dc.description.abstractPhthalates are widely used as plasticizers that influence sexual and reproductive development. Here, we investigated whether di-(2-ethylhexyl) phthalate (DEHP) affects macrophage polarization that are associated with tumor initiation and progression. No changes were observed in LPS- or ConA-stimulated in vitro spleen B or T cell proliferation for 48 h, respectively. In contrast, macrophage functions were inhibited in response to DEHP for 12 h as judged by LPS-induced H2O2 and NO production and zymosan A-mediated phagocytosis. When six weeks old male mice were pre-exposed to 4.0 mg/kg DEHP for 21 days before the injection of B16F10 melanoma cells and post-exposed to 4.0 mg/kg DEHP for 7 days, tumor nodule formation and the changes in tumor volume were higher than those in control group. Furthermore, when male mice were intraperitoneally pretreated with DEHP for 3 or 4 weeks and peritoneal exudate cells (PECs) or bone marrow-derived macrophages (BMDMs) were incubated with lipopolysaccharide (LPS), the expression of COX-2, TNF-alpha, and IL-6 was reduced in DEHP-pretreated cells as compared with that in LPS-stimulated control cells. While the production of nitric oxide (NO) for 18 h was reduced by LPS-stimulated PECs and M1-type BMDMs, IL-4 expression was enhanced in LPS-stimulated BMDMs. When BMDMs were incubated with IL-4 for 30 h, arginase 1 for M2-type macrophages was increased in transcriptional and translational level. Data implicate that macrophages were differentially polarized by DEHP treatment, which reduced M1-polarzation but enhanced M2-polarization. Taken together, these data demonstrate that DEHP could affect in vivo immune responses of macrophages, leading to the suppression of their tumor-preventing ability. This suggests that individuals at high risk for tumor incidence should avoid long-term exposure to various kind of phthalate including DEHP.en_US
dc.description.sponsorshipThis work was supported by Grant from Public Problem-Solving Program (NRF-015M3C8A6A06014500) and Mid-career Researcher Program (#2016-R1A2B400746) through the National Research Foundation (NRF) funded by the Ministry of Education, Science and Technology, Republic of Korea.en_US
dc.language.isoen_USen_US
dc.publisherELSEVIER SCI LTDen_US
dc.subjectDi-(2-ethylhexyl) phthalate (DEHP)en_US
dc.subjectMacrophage polarizationen_US
dc.subjectM1-typeen_US
dc.subjectM2-typeen_US
dc.subjectEndocrine disruptoren_US
dc.subjectTumor growthen_US
dc.titleDi-(2-ethylhexyl) phthalate enhances melanoma tumor growth via differential effect on M1-and M2-polarized macrophages in mouse modelen_US
dc.typeArticleen_US
dc.relation.volume233-
dc.identifier.doi10.1016/j.envpol.2017.10.030-
dc.relation.page833-843-
dc.relation.journalENVIRONMENTAL POLLUTION-
dc.contributor.googleauthorLee, Jae-Wook-
dc.contributor.googleauthorPark, Sojin-
dc.contributor.googleauthorHan, Hae-Kyoung-
dc.contributor.googleauthorGye, Myung Chan-
dc.contributor.googleauthorMoon, Eun-Yi-
dc.relation.code2018001199-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF NATURAL SCIENCES[S]-
dc.sector.departmentDEPARTMENT OF LIFE SCIENCE-
dc.identifier.pidmcgye-
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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