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Identification and characterization of human bone-derived cells

Title
Identification and characterization of human bone-derived cells
Author
황규태
Keywords
Human bone-derived cells; Osteoprogenitor cells; Undifferentiated preosteoblasts; Osteoblasts differentiation; Osteoblasts activity
Issue Date
2018-01
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v. 495, no. 1, page. 1257-1263
Abstract
This study was designed to identify and characterize primary bone-derived cells (BdCs) and investigate the potential role of osteoblast differentiation. Primary BdCs were isolated from surgical bone for comparative analysis with mesenchymal stem cells (MSCs) and fetal osteoblasts (FOBs) and for potential differentiation to mature osteoblasts. Using three different cells, we successfully cultivated human osteoblast differentiation and activity which were evaluated using microarray and biochemical methods. BdCs are more correlated to MSCs in bioinformatics result and similar with FOBs in gene expression. In particular, Osterix, osteoprogenitor marker, was high expressed in BdCs, while the expression in MSCs and FOBS were very low. Furthermore, BdCs exhibited a marked alkaline phosphatase (ALP) expression, early stage of osteogenic marker, and retained osteogenic properties and physiological changes into maturation as in FOBs. BdCs also showed an increase in bone morphogenic protein 2 (BMP2), osteopontin (OPN), and osteocalcin (OCN) mRNA expressions during differentiation. This study suggests that BdCs may be osteoprogenitor cells or undifferentiated preosteoblasts with strong capacity to differentiate toward mature osteoblasts. (C) 2017 Published by Elsevier Inc.
URI
https://www.sciencedirect.com/science/article/pii/S0006291X1732332X?via%3Dihubhttp://repository.hanyang.ac.kr/handle/20.500.11754/117261
ISSN
0006-291X; 1090-2104
DOI
10.1016/j.bbrc.2017.11.155
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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