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dc.contributor.author이민형-
dc.date.accessioned2019-12-04T00:59:56Z-
dc.date.available2019-12-04T00:59:56Z-
dc.date.issued2018-01-
dc.identifier.citationBIOMATERIALS SCIENCE, v. 6, no. 2, page. 407-417en_US
dc.identifier.issn2047-4830-
dc.identifier.issn2047-4849-
dc.identifier.urihttps://pubs.rsc.org/en/content/articlelanding/2018/BM/C7BM01088E#!divAbstract-
dc.identifier.urihttp://repository.hanyang.ac.kr/handle/20.500.11754/116951-
dc.description.abstractA glioblastoma is a common primary brain tumor that expresses microRNA- 21 (miR-21), which inhibits the expression of pro-apoptotic genes such as phosphatase and tensin homologue (PTEN) and programmed cell death 4 (PDCD4). Therefore, an antisense-oligonucleotide against miR-21 (miR21ASO) could have therapeutic effects for glioblastomas. In this study, curcumin was loaded into deoxycholic acid-conjugated polyethylenimine (DP) micelles. The curcumin-loaded DP micelle (DP-Cur) was evaluated as a carrier for the combined delivery of curcumin and miR21ASO. Gel retardation and heparin competition assays showed that DP-Cur formed stable complexes with miR21ASO. The anti-tumor effects of the combined delivery of curcumin and miR21ASO were evaluated in C6 glioblastoma cells. In vitro transfection showed that DP-Cur had an miR21ASO delivery efficiency similar to that of polyethylenimine (25 kDa, PEI25k) and DP. In the C6 cells, the delivery of miR21ASO using DP-Cur effectively reduced the miR21 level. The miR21ASO/DP-Cur complex induced apoptosis more effectively than the single delivery of curcumin or miR21ASO. The therapeutic effect of the miR21ASO/DP-Cur complex was also evaluated in an intracranial glioblastoma animal model. The miR21ASO/DP-Cur complex reduced the tumor volume more effectively than single therapy of curcumin or miR21ASO. Immunohistochemistry showed that PDCD4 and PTEN were induced in the miR21ASO/DP and miR21ASO/DP-Cur complex groups. Therefore, DP-Cur is an efficient carrier of miR21ASO and the combined delivery of miR21ASO and curcumin may be useful in the development of combination therapy for glioblastoma.en_US
dc.description.sponsorshipThis work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2016R1D1A1B03934778) and the Ministry of Science and ICT (NRF-2017R1A2B4009036). It was also supported by the Bio & Medical Technology Development Program through NRF funded by the Ministry of Science and ICT (NRF-2016M3A9B4918833).en_US
dc.language.isoen_USen_US
dc.publisherROYAL SOC CHEMISTRYen_US
dc.subjectGENE-THERAPYen_US
dc.subjectHEPATOCELLULAR-CARCINOMAen_US
dc.subjectENDOTHELIAL-CELLSen_US
dc.subjectPEPTIDE MICELLESen_US
dc.subjectHEME OXYGENASE-1en_US
dc.subjectCANCER-THERAPYen_US
dc.subjectDELIVERYen_US
dc.subjectBRAINen_US
dc.subjectAPOPTOSISen_US
dc.subjectOLIGODEOXYNUCLEOTIDEen_US
dc.titleA curcumin-loaded polymeric micelle as a carrier of a microRNA-21 antisense-oligonucleotide for enhanced anti-tumor effects in a glioblastoma animal modelen_US
dc.typeArticleen_US
dc.relation.no2-
dc.relation.volume6-
dc.identifier.doi10.1039/c7bm01088e-
dc.relation.page407-417-
dc.relation.journalBIOMATERIALS SCIENCE-
dc.contributor.googleauthorTan, Xiaonan-
dc.contributor.googleauthorKim, Gyeungyun-
dc.contributor.googleauthorLee, Dahee-
dc.contributor.googleauthorOh, Jungju-
dc.contributor.googleauthorKim, Minkyung-
dc.contributor.googleauthorPiao, Chunxian-
dc.contributor.googleauthorLee, Jaewon-
dc.contributor.googleauthorLee, Min Sang-
dc.contributor.googleauthorJeong, Ji Hoon-
dc.contributor.googleauthorLee, Minhyung-
dc.relation.code2018005650-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF ENGINEERING[S]-
dc.sector.departmentDEPARTMENT OF BIOENGINEERING-
dc.identifier.pidminhyung-
dc.identifier.orcidhttp://orcid.org/0000-0002-7083-9296-
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COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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