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Neural Stem Cell Death Mechanisms Induced by Amyloid Beta

Title
Neural Stem Cell Death Mechanisms Induced by Amyloid Beta
Author
고성호
Keywords
amyloid beta; neural stem cells; proteomics
Issue Date
2017-12
Publisher
대한치매학회
Citation
Dementia and Neurocognitive Disorders(대한치매학회지), v. 16, no. 4, page. 121-127
Abstract
Background and Purpose Amyloid beta (Aβ) is the main component of amyloid plaques, which are deposited in the brains of patients with Alzheimer’s disease (AD). Biochemical and animal studies support the central role of Aβ in AD pathogenesis. Despite several investigations focused on the pathogenic mechanisms of Aβ, it is still unclear how Aβ accumulates in the central nervous system and subsequently initiates the disease at the cellular level. In this study, we investigated the pathogenic mechanisms of Aβ using proteomics and antibody microarrays. Methods To evaluate the effect of Aβ on neural stem cells (NSCs), we treated primary cultured cortical NSCs with several doses of Aβ for 48 h. A 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay, trypan blue staining, and bromodeoxyuridine cell proliferation assay were performed. We detected several intracellular proteins that may be associated with Aβ by proteomics and Western blotting analysis. Results Various viability tests showed that Aβ decreased NSCs viability and cell proliferation in a concentration-dependent manner. Aβ treatment significantly decreased lactate dehydrogenase B, high-mobility group box 1, aldolase C, Ezrin, and survival signals including phosphorylated phosphoinositide 3-kinase, Akt, and glycogen synthase kinase-3β. Conclusions These results suggest that several factors determined by proteomics and Western blot hold the clue to Aβ pathogenesis. Further studies are required to investigate the role of these factors.
URI
https://dnd.or.kr/DOIx.php?id=10.12779/dnd.2017.16.4.121http://repository.hanyang.ac.kr/handle/20.500.11754/116821
ISSN
1738-1495; 2384-0757
DOI
10.12779/dnd.2017.16.4.121
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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