8 0

Granulocyte colony stimulating factor treatment in non-alcoholic fatty liver disease: Beyond marrow cell mobilization

Title
Granulocyte colony stimulating factor treatment in non-alcoholic fatty liver disease: Beyond marrow cell mobilization
Author
전대원
Keywords
non-alcoholic fatty liver; granulocyte colony stimulating factor; apoptosis
Issue Date
2017-12
Publisher
IMPACT JOURNALS LLC
Citation
ONCOTARGET, v. 8, no. 58, page. 97965-97976
Abstract
Protective effects of granulocyte colony stimulating factor (G-CSF) in acute liver injury via marrow cell mobilization have been reported in several studies. But exact mode of action and optimal protocol of G-CSF has been still doubt in chronic disease. Here we investigated mode of action and optimization of G-CSF as a treatment for nonalcoholic fatty liver disease (NAFLD). Various doses of conventional G-CSF (30 mu g/kg once weekly, once daily for 5 days, twice weekly) and long acting G-CSF (30 mu g/kg once a month) were evaluated in two kinds of NAFLD animal models to optimize the G-CSF protocol. G-CSF receptor expression highest increased in NAFLD model among various liver diseases compare to control (NAFLD: 14.7 times, alcohol hepatitis: 7.1 times, cirrhosis: 2.4 times, and ischemia reperfusion: 6.8 times). G-CSF treatment reduced intrahepatic fat accumulation, and inflammation in two kinds of NAFLD animal models. G-CSF increased PI3K/Akt expression in hepatocyte as well as decreased apoptotic drive (increased Bcl-2 expression and decreased Bax expression) in animal model. Five day consecutive G-CSF treatment and once a month long acting G-CSF increased marrow derived stem cell marker in peripheral blood. But twice a week conventional G-CSF treatment did not increased CD34+ cell in peripheral blood and liver neither. Not only high dose G-CSF (once daily for 5 days) but also hepatotropic dose G-CSF (twice a week) significantly reduced hepatocyte apoptosis via PI3K and Akt pathway activation without marrow cell mobilization in NAFLD animal model.
URI
http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=view&path[]=18967&path[]=60800http://repository.hanyang.ac.kr/handle/20.500.11754/116757
ISSN
1949-2553
DOI
10.18632/oncotarget.18967
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
Files in This Item:
Granulocyte colony stimulating factor treatment in non-alcoholic fatty liver disease beyond marrow cell mobilization.pdfDownload
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE