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dc.contributor.author채필석-
dc.date.accessioned2019-12-01T12:06:00Z-
dc.date.available2019-12-01T12:06:00Z-
dc.date.issued2017-10-
dc.identifier.citationANALYST, v. 142, no. 20, page. 3889-3898en_US
dc.identifier.issn0003-2654-
dc.identifier.issn1364-5528-
dc.identifier.urihttps://pubs.rsc.org/en/content/articlelanding/2017/AN/C7AN01168G#!divAbstract-
dc.identifier.urihttp://repository.hanyang.ac.kr/handle/20.500.11754/115827-
dc.description.abstractIntegral membrane proteins either alone or as complexes carry out a range of key cellular functions. Detergents are indispensable tools in the isolation of membrane proteins from biological membranes for downstream studies. Although a large number of techniques and tools, including a wide variety of detergents, are available, purification and structural characterization of many membrane proteins remain challenging. In the current study, a new class of tripod amphiphiles bearing two different penta-saccharide head groups, designated TPSs, were developed and evaluated for their ability to extract and stabilize a range of diverse membrane proteins. Variations in the structures of the detergent head and tail groups allowed us to prepare three sets of the novel agents with distinctive structures. Some TPSs (TPS-A8 and TPS-E7) were efficient at extracting two proteins in a functional state while others (TPS-E8 and TPS-E10L) conferred marked stability to all membrane proteins (and membrane protein complexes) tested here compared to a conventional detergent. Use of TPS-E10L led to clear visualization of a receptor-Gs complex using electron microscopy, indicating profound potential in membrane protein research.en_US
dc.description.sponsorshipThis work was supported by the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (grant number 2016R1A2B2011257 to P. S. C., M. E., and L. G.).en_US
dc.language.isoen_USen_US
dc.publisherROYAL SOC CHEMISTRYen_US
dc.subjectCRYSTAL-STRUCTUREen_US
dc.subjectFACIAL AMPHIPHILESen_US
dc.subjectMNG AMPHIPHILESen_US
dc.subjectCRYSTALLIZATIONen_US
dc.subjectSTABILIZATIONen_US
dc.subjectRECEPTORen_US
dc.subjectSOLUBILIZATIONen_US
dc.subjectDETERGENTSen_US
dc.subjectINSIGHTSen_US
dc.subjectBINDINGen_US
dc.titleNew penta-saccharide-bearing tripod amphiphiles for membrane protein structure studiesen_US
dc.typeArticleen_US
dc.relation.no20-
dc.relation.volume142-
dc.identifier.doi10.1039/c7an01168g-
dc.relation.page3889-3898-
dc.relation.journalANALYST-
dc.contributor.googleauthorEhsan, Muhammad-
dc.contributor.googleauthorGhani, Lubna-
dc.contributor.googleauthorDu, Yang-
dc.contributor.googleauthorHariharan, Parameswaran-
dc.contributor.googleauthorMortensen, Jonas S.-
dc.contributor.googleauthorRibeiro, Orquidea-
dc.contributor.googleauthorHu, Hongli-
dc.contributor.googleauthorSkiniotis, Georgios-
dc.contributor.googleauthorLoland, Claus J.-
dc.contributor.googleauthorGuan, Lan-
dc.contributor.googleauthorKobilka, Brian K.-
dc.contributor.googleauthorByrne, Bernadette-
dc.contributor.googleauthorChae, Pil Seok-
dc.relation.code2017002053-
dc.sector.campusS-
dc.sector.daehakGRADUATE SCHOOL[S]-
dc.sector.departmentDEPARTMENT OF BIONANOTECHNOLOGY-
dc.identifier.pidpchae-
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GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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