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A baseline drift detrending technique for fast scan cyclic voltammetry

Title
A baseline drift detrending technique for fast scan cyclic voltammetry
Author
김인영
Keywords
BACKGROUND SUBTRACTION; WAVE-FORM; DOPAMINE; MICROELECTRODES; BRAIN
Issue Date
2017-09
Publisher
ROYAL SOC CHEMISTRY
Citation
ANALYST, v. 142, no. 22, page. 4317-4321
Abstract
Fast scan cyclic voltammetry (FSCV) has been commonly used to measure extracellular neurotransmitter concentrations in the brain. Due to the unstable nature of the background currents inherent in FSCV measurements, analysis of FSCV data is limited to very short amounts of time using traditional background subtraction. In this paper, we propose the use of a zero-phase high pass filter (HPF) as the means to remove the background drift. Instead of the traditional method of low pass filtering across voltammograms to increase the signal to noise ratio, a HPF with a low cutoff frequency was applied to the temporal dataset at each voltage point to remove the background drift. As a result, the HPF utilizing cutoff frequencies between 0.001 Hz and 0.01 Hz could be effectively used to a set of FSCV data for removing the drifting patterns while preserving the temporal kinetics of the phasic dopamine response recorded in vivo. In addition, compared to a drift removal method using principal component analysis, this was found to be significantly more effective in reducing the drift (unpaired t-test p < 0.0001, t = 10.88) when applied to data collected from Tris buffer over 24 hours although a drift removal method using principal component analysis also showed the effective background drift reduction. The HPF was also applied to 5 hours of FSCV in vivo data. Electrically evoked dopamine peaks, observed in the nucleus accumbens, were clearly visible even without background subtraction. This technique provides a new, simple, and yet robust, approach to analyse FSCV data with an unstable background.
URI
https://pubs.rsc.org/en/content/articlelanding/2017/AN/C7AN01465A#!divAbstracthttp://repository.hanyang.ac.kr/handle/20.500.11754/115458
ISSN
0003-2654; 1364-5528
DOI
10.1039/c7an01465a
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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