Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이민형 | - |
dc.date.accessioned | 2019-11-27T20:11:19Z | - |
dc.date.available | 2019-11-27T20:11:19Z | - |
dc.date.issued | 2017-07 | - |
dc.identifier.citation | PHARMACEUTICAL RESEARCH, v. 34, no. 7, page. 1517-1526 | en_US |
dc.identifier.issn | 0724-8741 | - |
dc.identifier.issn | 1573-904X | - |
dc.identifier.uri | https://link.springer.com/article/10.1007%2Fs11095-017-2175-6 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/114967 | - |
dc.description.abstract | Purpose Adiponectin (APN) is an adipokine with antiinflammatory and cytoprotective effects. In this study, the therapeutic effect of APN gene delivery using a polymeric carrier was evaluated in an acute lung injury (ALI) model. Methods Polyethylenimine (2 kDa, PEI2K), PEI25K (25 kDa), polyamidoamine (generation 2, PAMG2), dexamethasone-conjugated PEI2k (PEI2K-Dexa), and dexamethasone-conjugated PAMG2 (PAMG2-Dexa) were evaluated in vitro and in vivo as gene carriers. Formation of plasmid DNA (pDNA)/carrier complexes was confirmed by gel retardation and heparin competition assays. Delivery efficiency was measured by a luciferase assay and fluorescence microscopy. In an ALI animal model, pAPN/carrier complexes were delivered by intratracheal administration. Therapeutic effects were evaluated by cytokine assays and hematoxylin and eosin (H&E) staining. Results Gel retardation assays showed that PEI2K-Dexa and PAMG2-Dexa formed complexes with pDNA. In L2 lung epithelial cells, PAMG2-Dexa yielded higher transfection efficiency than PEI2K, PAMG2, PEI25K, lipofectamine, and PEI2K-Dexa. In vivo experiments showed that PAMG2-Dexa delivered DNA more efficiently to lung tissue than PEI2KDexa and PEI25K. Delivery of pAPN/PAMG2-Dexa complexes upregulated APN expression in the lungs of mice with ALI. As a result, the levels of pro-inflammatory cytokines such as TNF-alpha and IL-1 beta were decreased. H&E staining showed that inflammation in the lungs of mice with ALI was reduced by delivery of the APN gene. Conclusion Delivery of the APN gene using PAMG2-Dexa reduced inflammation in the lungs of mice with ALI. The APN gene could be a useful tool in the development of gene therapy for ALI. | en_US |
dc.description.sponsorship | This work was supported by a grant from the Ministry of Health and Welfare in Korea (HI12C1210). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | SPRINGER/PLENUM PUBLISHERS | en_US |
dc.subject | Acute lung injury | en_US |
dc.subject | Adiponectin | en_US |
dc.subject | Gene delivery | en_US |
dc.subject | Inflammation | en_US |
dc.subject | Polymeric carrier | en_US |
dc.title | Anti-Inflammatory Therapeutic Effect of Adiponectin Gene Delivery Using a Polymeric Carrier in an Acute Lung Injury Model | en_US |
dc.type | Article | en_US |
dc.relation.no | 7 | - |
dc.relation.volume | 34 | - |
dc.identifier.doi | 10.1007/s11095-017-2175-6 | - |
dc.relation.page | 1517-1526 | - |
dc.relation.journal | PHARMACEUTICAL RESEARCH | - |
dc.contributor.googleauthor | Piao, Chunxian | - |
dc.contributor.googleauthor | Park, Jeong Hyun | - |
dc.contributor.googleauthor | Lee, Minhyung | - |
dc.relation.code | 2017000773 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | minhyung | - |
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