DNA-free Genome Editing of Chlamydomonas reinhardtii Using CRISPR and Subsequent Mutant Analysis
- Title
- DNA-free Genome Editing of Chlamydomonas reinhardtii Using CRISPR and Subsequent Mutant Analysis
- Author
- 진언선
- Keywords
- Genome editing; CRISPR-Cas9; Microalgae; Ribonucleoproteins; Chlamydomonas reinhardtii; DNA-free transformation
- Issue Date
- 2017-06
- Publisher
- BIO-PROTOCOL
- Citation
- BIO-PROTOCOL, v. 7, no. 11, Article no. e2352
- Abstract
- We successfully introduced targeted knock-out of gene of interest in Chlamydomonas reinhardtii by using DNA-free CRISPR. In this protocol, the detailed procedures of an entire workflow cover from the initial target selection of CRISPR to the mutant analysis using next generation sequencing (NGS) technology. Furthermore, we introduce a web-based set of tools, named CRISPR RGEN tools (http://www.rgenome.net/), which provides all required tools from CRISPR target design to NGS data analysis.
- URI
- https://bio-protocol.org/e2352https://repository.hanyang.ac.kr/handle/20.500.11754/114514
- ISSN
- 2331-8325
- DOI
- 10.21769/BioProtoc.2352
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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