Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 황승용 | - |
dc.date.accessioned | 2019-11-26T01:35:03Z | - |
dc.date.available | 2019-11-26T01:35:03Z | - |
dc.date.issued | 2017-06 | - |
dc.identifier.citation | BIOCHIP JOURNAL, v. 11, no. 2, page. 109-115 | en_US |
dc.identifier.issn | 1976-0280 | - |
dc.identifier.issn | 2092-7843 | - |
dc.identifier.uri | https://link.springer.com/article/10.1007%2Fs13206-016-1203-7 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/114404 | - |
dc.description.abstract | This paper presents a proof-of-concept microfluidic aptamer-based sensor for thrombin point of -care testing using electrochemical impedance spectroscopy. The disposable biosensor is composed of a polydimethylsiloxane (PDMS) channel layer over a glass substrate. The substrate surface has an Au working electrode and a Pt reference/counter electrode. In this study, human thrombin was used as a biomarker for disease diagnosis. An unlabeled aptamer specific to thrombin was immobilized on a working electrode and electrochemical impedance was measured as thrombin was injected into the biosensor. Thrombin was detected by measuring change in impedance. The proposed biochip had a detection range of 0.1-100,000 ng mL(-1) for thrombin concentration and a limit of detection of 0.1 ng mL(-1). The results indicated that our biochip could be an effective tool for other molecular diagnostic systems. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | KOREAN BIOCHIP SOCIETY-KBCS | en_US |
dc.subject | DNA Aptamer | en_US |
dc.subject | Microfluidics | en_US |
dc.subject | Thrombin | en_US |
dc.subject | Electrochemical impedance spectroscopy | en_US |
dc.subject | Anti-thrombin aptamer | en_US |
dc.title | Microfluidic biochips for simple impedimetric detection of thrombin based on label-free DNA aptamers | en_US |
dc.type | Article | en_US |
dc.relation.no | 2 | - |
dc.relation.volume | 11 | - |
dc.identifier.doi | 10.1007/s13206-016-1203-7 | - |
dc.relation.page | 109-115 | - |
dc.relation.journal | BIOCHIP JOURNAL | - |
dc.contributor.googleauthor | Limi, Taemin | - |
dc.contributor.googleauthor | Lee, Seung Yong | - |
dc.contributor.googleauthor | Yang, Jeongsuong | - |
dc.contributor.googleauthor | Hwang, Seung Yong | - |
dc.contributor.googleauthor | Ahn, Yoomin | - |
dc.relation.code | 2017004186 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | syhwang | - |
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