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Tandem malonate-based glucosides (TMGs) for membrane protein structural studies

Title
Tandem malonate-based glucosides (TMGs) for membrane protein structural studies
Author
채필석
Keywords
FACIAL AMPHIPHILES; MELIBIOSE PERMEASE; ESCHERICHIA-COLI; STABILIZATION; SOLUBILIZATION; DETERGENTS; CRYSTALLIZATION; BINDING; UAPA; MECHANISMS
Issue Date
2017-06
Publisher
NATURE PUBLISHING GROUP
Citation
SCIENTIFIC REPORTS, v. 7, Article no. 3963
Abstract
High-resolution membrane protein structures are essential for understanding the molecular basis of diverse biological events and important in drug development. Detergents are usually used to extract these bio-macromolecules from the membranes and maintain them in a soluble and stable state in aqueous solutions for downstream characterization. However, many eukaryotic membrane proteins solubilized in conventional detergents tend to undergo structural degradation, necessitating the development of new amphiphilic agents with enhanced properties. In this study, we designed and synthesized a novel class of glucoside amphiphiles, designated tandem malonate-based glucosides (TMGs). A few TMG agents proved effective at both stabilizing a range of membrane proteins and extracting proteins from the membrane environment. These favourable characteristics, along with synthetic convenience, indicate that these agents have potential in membrane protein research.
URI
https://www.nature.com/articles/s41598-017-03809-3http://repository.hanyang.ac.kr/handle/20.500.11754/114401
ISSN
2045-2322
DOI
10.1038/s41598-017-03809-3
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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