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DROSHA targets its own transcript to modulate alternative splicing

Title
DROSHA targets its own transcript to modulate alternative splicing
Author
남진우
Keywords
DROSHA; Microprocessor; alternative splicing
Issue Date
2017-04
Publisher
COLD SPRING HARBOR LAB PRESS
Citation
RNA, v. 23, no. 7, page. 1035-1047
Abstract
The nuclear RNase Ill enzyme DROSHA interacts with its cofactor DGCR8 to form the Microprocessor complex, which initiates microRNA (miRNA) maturation by cleaving hairpin structures embedded in primary transcripts. Apart from its central role in the biogenesis of miRNAs, DROSHA is also known to recognize and cleave miRNA-like hairpins in a subset of transcripts without apparent small RNA production. Here, we report that the human DROSHA transcript is one such noncanonical target of DROSHA. Mammalian DROSHA genes have evolved a conserved hairpin structure spanning a specific exon-intron junction, which serves as a substrate for the Microprocessor in human cells but not in murine cells. We show that it is this hairpin element that decides whether the overlapping exon is alternatively or constitutively spliced. We further demonstrate that DROSHA promotes skipping of the overlapping exon in human cells independently of its cleavage function. Our findings add to the expanding list of noncanonical DROSHA functions.
URI
https://rnajournal.cshlp.org/content/23/7/1035http://repository.hanyang.ac.kr/handle/20.500.11754/113728
ISSN
1355-8382; 1469-9001
DOI
10.1261/rna.059808.116
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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