From single-molecule detection to next-generation sequencing: microfluidic droplets for high-throughput nucleic acid analysis
- Title
- From single-molecule detection to next-generation sequencing: microfluidic droplets for high-throughput nucleic acid analysis
- Author
- 주재범
- Keywords
- Microfluidic; Droplets; Digital PCR; Nextgeneration sequencing; Single-cell RNA sequencing; Diagnostics
- Issue Date
- 2017-03
- Publisher
- SPRINGER HEIDELBERG
- Citation
- MICROFLUIDICS AND NANOFLUIDICS, v. 21, no. 3
- Abstract
- Droplet-based microfluidic technologies have proved themselves to be of significant utility in the performance of high-throughput chemical and biological experiments. By encapsulating and isolating reagents within femtoliter-nanoliter droplet, millions of (bio) chemical reactions can be processed in a parallel fashion and on ultra-short timescales. Recent applications of such technologies to genetic analysis have suggested significant utility in low-cost, efficient and rapid workflows for DNA amplification, rare mutation detection, antibody screening and next-generation sequencing. To this end, we describe and highlight some of the most interesting recent developments and applications of droplet-based microfluidics in the broad area of nucleic acid analysis. In addition, we also present a cursory description of some of the most essential functional components, which allow the creation of integrated and complex workflows based on flowing streams of droplets.
- URI
- https://link.springer.com/article/10.1007%2Fs10404-017-1889-4https://repository.hanyang.ac.kr/handle/20.500.11754/113387
- ISSN
- 1613-4982; 1613-4990
- DOI
- 10.1007/s10404-017-1889-4
- Appears in Collections:
- GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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