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ESR1 inhibits hCG-induced steroidogenesis and proliferation of progenitor Leydig cells in mice

Title
ESR1 inhibits hCG-induced steroidogenesis and proliferation of progenitor Leydig cells in mice
Author
계명찬
Keywords
ESTROGEN-RECEPTOR-ALPHA; HUMAN CHORIONIC-GONADOTROPIN; MALE REPRODUCTIVE-TRACT; FETAL-RAT TESTIS; ANDROGEN BIOSYNTHESIS; NEONATAL EXPOSURE; GENE-EXPRESSION; MOUSE TESTIS; DIFFERENTIATION; BETA
Issue Date
2017-03
Publisher
NATURE PUBLISHING GROUP
Citation
SCIENTIFIC REPORTS, v. 7, Article no. 43459
Abstract
Oestrogen is an important regulator in reproduction. To understand the role of oestrogen receptor 1 (ESR1) in Leydig cells, we investigated the expression of ESR1 in mouse Leydig cells during postnatal development and the effects of oestrogen on steroidogenesis and proliferation of progenitor Leydig cells (PLCs). In Leydig cells, the ESR1 expression was low at birth, increased until postnatal day 14 at which PLCs were predominant, and then decreased until adulthood. In foetal Leydig cells, ESR1 immunoreactivity increased from birth to postnatal day 14. These suggest that ESR1 is a potential biomarker of Leydig cell development. In PLCs, 17 beta-estradiol and the ESR1-selective agonist propylpyrazoletriol suppressed human chorionic gonadotropin (hCG)-induced progesterone production and steroidogenic gene expression. The ESR2-selective agonist diarylpropionitrile did not affect steroidogenesis. In PLCs from Esr1 knockout mice, hCG-stimulated steroidogenesis was not suppressed by 17 beta-estradiol, suggesting that oestrogen inhibits PLC steroidogenesis via ESR1. 17 beta-estradiol, propylpyrazoletriol, and diarylpropionitrile decreased bromodeoxyuridine uptake in PLCs in the neonatal mice. In cultured PLCs, 17 beta-estradiol, propylpyrazoletriol, and diarylpropionitrile reduced hCG-stimulated Ki67 and Pcna mRNA expression and the number of KI67-positive PLCs, suggesting that oestrogen inhibits PLC proliferation via both ESR1 and ESR2. In PLCs, ESR1 mediates the oestrogen-induced negative regulation of steroidogenesis and proliferation.
URI
https://www.nature.com/articles/srep43459http://repository.hanyang.ac.kr/handle/20.500.11754/113304
ISSN
2045-2322
DOI
10.1038/srep43459
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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