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Transcriptome analysis shows ambiguous phenotypes of murine primitive endoderm-related stem cell lines

Title
Transcriptome analysis shows ambiguous phenotypes of murine primitive endoderm-related stem cell lines
Author
Binas, Bert
Keywords
extraembryonic endoderm; mice; nEnd; primitive endoderm (PrE); pXEN; XEN
Issue Date
2019-04
Publisher
WILEY
Citation
GENES TO CELLS, v. 24, No. 4, Page. 324-331
Abstract
Primitive endoderm (PrE)-related cell lines (XEN, pXEN and nEnd cells) show key features of the PrE. By transcriptome analysis, we show: (a) Compared to embryonic stem cells, PrE-related cell lines are less in vivo like, although early nEnd cells are most similar to the PrE. (b) These cell lines show post-PrE features of parietal (XEN and pXEN cells) or visceral (nEnd cells) endoderm, likely driven by Tgf-beta and Wnt/Activin signaling, respectively. (c) pXEN and nEnd cell lines additionally show pre-PrE features. Hence, neither pXEN nor nEnd cell cultures represent a distinct in vivo entity. Rather, their properties are compatible with mixed and hybrid phenotypes. Our findings indicate that pre-PrE, PrE and early post-PrE phenotypes result from different niches, which need to be better understood to derive cell lines that distinctly represent the early stages of the extraembryonic endoderm.
URI
https://onlinelibrary.wiley.com/doi/full/10.1111/gtc.12678http://repository.hanyang.ac.kr/handle/20.500.11754/113136
ISSN
1356-9597; 1365-2443
DOI
10.1111/gtc.12678
Appears in Collections:
COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > MOLECULAR AND LIFE SCIENCE(분자생명과학과) > Articles
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