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dc.contributor.author윤태현-
dc.date.accessioned2019-11-20T12:16:10Z-
dc.date.available2019-11-20T12:16:10Z-
dc.date.issued2017-02-
dc.identifier.citationANALYTICAL CHEMISTRY, v. 89, no. 4, page. 2449-2456en_US
dc.identifier.issn0003-2700-
dc.identifier.issn1520-6882-
dc.identifier.urihttps://pubs.acs.org/doi/10.1021/acs.analchem.6b04418-
dc.identifier.urihttp://repository.hanyang.ac.kr/handle/20.500.11754/112817-
dc.description.abstractThere has been a great deal of research regarding the cellular association of nanoparticles (NPs), although there are only a few methods available yet for the quantitative measurements of cellular NPs. In this study, we propose a simple and quantitative method to estimate the cellular uptake of Au NPs into cervical cancer cells (HeLa) based on their side scattering (SSC) intensities measured by flow cytometry (FCM). We have compared SSC intensities of HeLa cells exposed to eight different types of Au NPs (40-100 nm size, with positive or negative surface charge) with the amount of cellular Au NPs measured by inductively coupled plasma mass spectrometry (ICPMS). On the basis of these comparisons, we have found linear correlations between the cellular Au NPs and the SSC intensities and used them to estimate the amount of Au NPs associated with HeLa cells. Once the correlations were found for specific cell lines and types of nanoparticles, this approach is useful for simple and quantitative estimation of the cellular Au NPs, without performing labor-intensive and complicated sample preparation procedures required for the ICPMS approach.en_US
dc.description.sponsorshipThis work was supported by the Industrial Strategic Technology Development Program (Grant 10043929, Development of "User-friendly Nanosafety Prediction System"), funded by the Ministry of Trade, Industry & Energy (MOTIE) of Korea. We also appreciate Desy Maulina for proofreading of the manuscript and Julie J. Yoon for assistance in improving the TOC graphic quality.en_US
dc.language.isoen_USen_US
dc.publisherAMER CHEMICAL SOCen_US
dc.subjectGOLD NANOPARTICLESen_US
dc.subjectPROTEIN ADSORPTIONen_US
dc.subjectMAMMALIAN-CELLSen_US
dc.subjectSURFACE-CHARGEen_US
dc.subjectLIGHT SCATTERen_US
dc.subjectSIZEen_US
dc.subjectFLUORESCENCEen_US
dc.subjectENDOCYTOSISen_US
dc.subjectTOXICITYen_US
dc.titleFlow Cytometry-Based Quantification of Cellular Au Nanoparticlesen_US
dc.typeArticleen_US
dc.relation.no4-
dc.relation.volume89-
dc.identifier.doi10.1021/acs.analchem.6b04418-
dc.relation.page2449-2456-
dc.relation.journalANALYTICAL CHEMISTRY-
dc.contributor.googleauthorPark, Jonghoon-
dc.contributor.googleauthorHa, My Kieu-
dc.contributor.googleauthorYang, Nuri-
dc.contributor.googleauthorYoon, Tae Hyun-
dc.relation.code2017002986-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF NATURAL SCIENCES[S]-
dc.sector.departmentDEPARTMENT OF CHEMISTRY-
dc.identifier.pidtaeyoon-
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > CHEMISTRY(화학과) > Articles
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