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Flow Cytometry-Based Quantification of Cellular Au Nanoparticles

Title
Flow Cytometry-Based Quantification of Cellular Au Nanoparticles
Author
윤태현
Keywords
GOLD NANOPARTICLES; PROTEIN ADSORPTION; MAMMALIAN-CELLS; SURFACE-CHARGE; LIGHT SCATTER; SIZE; FLUORESCENCE; ENDOCYTOSIS; TOXICITY
Issue Date
2017-02
Publisher
AMER CHEMICAL SOC
Citation
ANALYTICAL CHEMISTRY, v. 89, no. 4, page. 2449-2456
Abstract
There has been a great deal of research regarding the cellular association of nanoparticles (NPs), although there are only a few methods available yet for the quantitative measurements of cellular NPs. In this study, we propose a simple and quantitative method to estimate the cellular uptake of Au NPs into cervical cancer cells (HeLa) based on their side scattering (SSC) intensities measured by flow cytometry (FCM). We have compared SSC intensities of HeLa cells exposed to eight different types of Au NPs (40-100 nm size, with positive or negative surface charge) with the amount of cellular Au NPs measured by inductively coupled plasma mass spectrometry (ICPMS). On the basis of these comparisons, we have found linear correlations between the cellular Au NPs and the SSC intensities and used them to estimate the amount of Au NPs associated with HeLa cells. Once the correlations were found for specific cell lines and types of nanoparticles, this approach is useful for simple and quantitative estimation of the cellular Au NPs, without performing labor-intensive and complicated sample preparation procedures required for the ICPMS approach.
URI
https://pubs.acs.org/doi/10.1021/acs.analchem.6b04418http://repository.hanyang.ac.kr/handle/20.500.11754/112817
ISSN
0003-2700; 1520-6882
DOI
10.1021/acs.analchem.6b04418
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > CHEMISTRY(화학과) > Articles
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