Non-ionic detergents Nonidet P-40 and Triton X-100 increase enzymatic activity of plasmin
- Title
- Non-ionic detergents Nonidet P-40 and Triton X-100 increase enzymatic activity of plasmin
- Author
- 이철훈
- Keywords
- Non-ionic; Detergent; Enzyme; Plasmin; Non-competitive; Stimulator
- Issue Date
- 2019-04
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v. 512, No. 2, Page. 314-318
- Abstract
- Plasmin is a potent serin protease involved in a variety of biological functions, such as fibrinolysis and tissue remodeling. On performing an in vitro control assay to measure the activity of endogenous plasmin in cell lysates, a stimulatory effect of non-ionic detergent NP-40 on plasmin activity was discovered. Another non-ionic detergent, TX-100, also enhanced plasmin activity, while ionic detergents sodium deoxycholate and sodiem dodecyl sulfate abolished plasmin enzyme activity. Kinetic analysis of plasmin activity in the presence of NP-40 and TX-100 demonstrated an increase in V-max; however, there was no change in K-m values, suggesting that these detergents stimulate plasmin activity in a non-competitive manner. Fibrin plate assay indicates that NP-40 and TX-100 functionally stimulate plasmin activity by showing a dose-dependent increase in fibrinolysis. (C) 2019 Elsevier Inc. All rights reserved.
- URI
- https://www.sciencedirect.com/science/article/pii/S0006291X19304292https://repository.hanyang.ac.kr/handle/20.500.11754/112537
- ISSN
- 0006-291X; 1090-2104
- DOI
- 10.1016/j.bbrc.2019.03.052
- Appears in Collections:
- COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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