Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 임형신 | - |
dc.date.accessioned | 2019-11-19T07:27:18Z | - |
dc.date.available | 2019-11-19T07:27:18Z | - |
dc.date.issued | 2019-02 | - |
dc.identifier.citation | PLANTA MEDICA, v. 85, No. 3, Page. 217-224 | en_US |
dc.identifier.issn | 0032-0943 | - |
dc.identifier.issn | 1439-0221 | - |
dc.identifier.uri | https://www.thieme-connect.com/products/ejournals/html/10.1055/a-0731-0394 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/112437 | - |
dc.description.abstract | Polo-like kinase 1, a mitotic Ser/Thr kinase, has emerged as a molecular target for the development of anticancer drugs. In this study, we found that polo-like kinase 1 activity was inhibited by 7- O -methylwogonin and related flavones, including baicalein, dihydrobaicalein, and viscidulin II, isolated from Scutellaria baicalensis . Although dihydrobaicalein exhibited the highest polo-like kinase 1 inhibitory activity among the four compounds, it also inhibited other kinases, such as vaccinia-related kinase 2 and polo-like kinase 2. Baicalein and viscidulin II also showed low selectivity to polo-like kinase 1 since they inhibited polo-like kinase 3 and polo-like kinase 2, respectively. However, 7- O -methylwogonin exhibited selective polo-like kinase 1 inhibitory activity, as evidenced from in vitro kinase assays based on fluorescence resonance energy transfer assays and ADP-Glo kinase assays. In addition, examination of mitotic morphology and immunostaining using specific antibodies for the mitotic markers, p-histone H3 and mitotic protein monoclonal 2, in Hep3B cells showed that 7- O -methylwogonin treatment increased mitotic cell populations due to inhibition of mitotic progression as a result of polo-like kinase 1 inhibition. The pattern of 7- O -methylwogonin-induced mitotic arrest was similar to that of BI 2536, a specific polo-like kinase 1 inhibitor. Thus, it was suggested that 7- O -methylwogonin disturbed mitotic progression by inhibiting polo-like kinase 1 activity. These data suggest that 7- O -methylwogonin, a polo-like kinase 1 inhibitor, may be a useful anticancer agent because of its polo-like kinase 1 selectivity and effectiveness. | en_US |
dc.description.sponsorship | This work was supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2014R1A2A1A11049701, NRF-2017R1A2B2012301 to H. Y.). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | GEORG THIEME VERLAG KG | en_US |
dc.subject | 7-O-methylwogonin | en_US |
dc.subject | polo-like kinase 1 | en_US |
dc.subject | Scutellaria baicalensis | en_US |
dc.subject | Lamiaceae | en_US |
dc.subject | anticancer | en_US |
dc.subject | mitosis | en_US |
dc.title | 7-O -Methylwogonin from Scutellaria baicalensis Disturbs Mitotic Progression by Inhibiting Plk1 Activity in Hep3B Cells | en_US |
dc.type | Article | en_US |
dc.relation.no | 3 | - |
dc.relation.volume | 85 | - |
dc.identifier.doi | 10.1055/a-0731-0394 | - |
dc.relation.page | 217-224 | - |
dc.relation.journal | PLANTA MEDICA | - |
dc.contributor.googleauthor | Woo, Sang-Uk | - |
dc.contributor.googleauthor | Jang, Hay-Ran | - |
dc.contributor.googleauthor | Chin, Young-Won | - |
dc.contributor.googleauthor | Yim, Hyungshin | - |
dc.relation.code | 2019002028 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF PHARMACY[E] | - |
dc.sector.department | DEPARTMENT OF PHARMACY | - |
dc.identifier.pid | hsyim | - |
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