27 0

Full metadata record

DC FieldValueLanguage
dc.contributor.author임형신-
dc.date.accessioned2019-11-19T07:27:18Z-
dc.date.available2019-11-19T07:27:18Z-
dc.date.issued2019-02-
dc.identifier.citationPLANTA MEDICA, v. 85, No. 3, Page. 217-224en_US
dc.identifier.issn0032-0943-
dc.identifier.issn1439-0221-
dc.identifier.urihttps://www.thieme-connect.com/products/ejournals/html/10.1055/a-0731-0394-
dc.identifier.urihttp://repository.hanyang.ac.kr/handle/20.500.11754/112437-
dc.description.abstractPolo-like kinase 1, a mitotic Ser/Thr kinase, has emerged as a molecular target for the development of anticancer drugs. In this study, we found that polo-like kinase 1 activity was inhibited by 7- O -methylwogonin and related flavones, including baicalein, dihydrobaicalein, and viscidulin II, isolated from Scutellaria baicalensis . Although dihydrobaicalein exhibited the highest polo-like kinase 1 inhibitory activity among the four compounds, it also inhibited other kinases, such as vaccinia-related kinase 2 and polo-like kinase 2. Baicalein and viscidulin II also showed low selectivity to polo-like kinase 1 since they inhibited polo-like kinase 3 and polo-like kinase 2, respectively. However, 7- O -methylwogonin exhibited selective polo-like kinase 1 inhibitory activity, as evidenced from in vitro kinase assays based on fluorescence resonance energy transfer assays and ADP-Glo kinase assays. In addition, examination of mitotic morphology and immunostaining using specific antibodies for the mitotic markers, p-histone H3 and mitotic protein monoclonal 2, in Hep3B cells showed that 7- O -methylwogonin treatment increased mitotic cell populations due to inhibition of mitotic progression as a result of polo-like kinase 1 inhibition. The pattern of 7- O -methylwogonin-induced mitotic arrest was similar to that of BI 2536, a specific polo-like kinase 1 inhibitor. Thus, it was suggested that 7- O -methylwogonin disturbed mitotic progression by inhibiting polo-like kinase 1 activity. These data suggest that 7- O -methylwogonin, a polo-like kinase 1 inhibitor, may be a useful anticancer agent because of its polo-like kinase 1 selectivity and effectiveness.en_US
dc.description.sponsorshipThis work was supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2014R1A2A1A11049701, NRF-2017R1A2B2012301 to H. Y.).en_US
dc.language.isoen_USen_US
dc.publisherGEORG THIEME VERLAG KGen_US
dc.subject7-O-methylwogoninen_US
dc.subjectpolo-like kinase 1en_US
dc.subjectScutellaria baicalensisen_US
dc.subjectLamiaceaeen_US
dc.subjectanticanceren_US
dc.subjectmitosisen_US
dc.title7-O -Methylwogonin from Scutellaria baicalensis Disturbs Mitotic Progression by Inhibiting Plk1 Activity in Hep3B Cellsen_US
dc.typeArticleen_US
dc.relation.no3-
dc.relation.volume85-
dc.identifier.doi10.1055/a-0731-0394-
dc.relation.page217-224-
dc.relation.journalPLANTA MEDICA-
dc.contributor.googleauthorWoo, Sang-Uk-
dc.contributor.googleauthorJang, Hay-Ran-
dc.contributor.googleauthorChin, Young-Won-
dc.contributor.googleauthorYim, Hyungshin-
dc.relation.code2019002028-
dc.sector.campusE-
dc.sector.daehakCOLLEGE OF PHARMACY[E]-
dc.sector.departmentDEPARTMENT OF PHARMACY-
dc.identifier.pidhsyim-
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE