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dc.contributor.author전대원-
dc.date.accessioned2019-11-19T05:18:45Z-
dc.date.available2019-11-19T05:18:45Z-
dc.date.issued2017-01-
dc.identifier.citationTOXICOLOGY AND APPLIED PHARMACOLOGY, v. 316, npage. 74-82en_US
dc.identifier.issn0041-008X-
dc.identifier.issn1096-0333-
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0041008X16303957?via%3Dihub-
dc.identifier.urihttp://repository.hanyang.ac.kr/handle/20.500.11754/112260-
dc.description.abstractEmerging evidence has shown that berberine has a protective effect against metabolic syndrome such as obesity and type II diabetes mellitus by activating AMP-activated protein kinase (AMPK). AMPK induces CD36 trafficking to the sarcolemma for fatty acid uptake and oxidation in contracting muscle. However, little is known about the effects of AMPK on CD36 regulation in the liver. We investigated whether AMPK activation by berberine affects CD36 expression and fatty acid uptake in hepatocytes and whether it is linked to hepatic lipid accumulation. Activation of AMPK by berberine or transduction with adenoviral vectors encoding constitutively active AMPK in HepG2 and mouse primary hepatocytes increased the expression and membrane translocation of CD36, resulting in enhanced fatty acid uptake and lipid accumulation as determined by BODIPY-C16 and Nile red fluorescence, respectively. Activation of AMPK by berberine induced the phosphorylation of extracellular signal-regulated kinases 1/2 (ERK1/2) and subsequently induced CCAAT/enhancer-binding protein beta (C/EBP beta) binding to the C/EBP-response element in the CD36 promoter in hepatocytes. In addition, hepatic CD36 expression and triglyceride levels were increased in normal diet-fed mice treated with berberine, but completely prevented when hepatic CD36 was silenced with adenovirus containing CD36-specific shRNA. Taken together, prolonged activation of AMPK by berberine increased CD36 expression in hepatocytes, resulting in fatty acid uptake via processes linked to hepatocellular lipid accumulation and fatty liver. (C) 2016 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipThis research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP; No. 2007-0056817) and the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HI14C0133).en_US
dc.language.isoenen_US
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCEen_US
dc.subjectBerberineen_US
dc.subjectAMPKen_US
dc.subjectAICARen_US
dc.subjectCD36en_US
dc.subjectFatty liveren_US
dc.subjectFatty acid uptakeen_US
dc.titleActivation of AMPK by berberine induces hepatic lipid accumulation by upregulation of fatty acid translocase CD36 in miceen_US
dc.typeArticleen_US
dc.relation.volume316-
dc.identifier.doi10.1016/j.taap.2016.12.019-
dc.relation.page74-82-
dc.relation.journalTOXICOLOGY AND APPLIED PHARMACOLOGY-
dc.contributor.googleauthorChoi, You-Jin-
dc.contributor.googleauthorLee, Kang-Yo-
dc.contributor.googleauthorJung, Seung-Hwan-
dc.contributor.googleauthorKim, Hyung Sik-
dc.contributor.googleauthorShim, Gayong-
dc.contributor.googleauthorKim, Mi-Gyeong-
dc.contributor.googleauthorOh, Yu-Kyoung-
dc.contributor.googleauthorOh, Seon-Hee-
dc.contributor.googleauthorJun, Dae Won-
dc.contributor.googleauthorLee, Byung-Hoon-
dc.relation.code2017001831-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF MEDICINE[S]-
dc.sector.departmentDEPARTMENT OF MEDICINE-
dc.identifier.pidnoshin-
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