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Yeast symmetric arginine methyltransferase Hsl7 has a repressive role in transcription

Title
Yeast symmetric arginine methyltransferase Hsl7 has a repressive role in transcription
Author
안성훈
Keywords
Symmetric arginine methylation; Hsl7; Histone deacetylation; Rpd3; Transcriptional repression
Issue Date
2019-06
Publisher
ELSEVIER SCIENCE BV
Citation
RESEARCH IN MICROBIOLOGY, v. 170, No. 4-5, Page. 222-229
Abstract
Protein arginine methylation, an evolutionarily conserved post-translational modification, serves critical cellular functions by transferring a methyl group to a variety of substrates, including histones and some transcription factors. In budding yeast, Hsl7 (histone synthetic lethal 7) displays type II PRMT (protein arginine methyltransferase) activity by generating symmetric dimethylarginine residues on histone H2A in vitro. However, identification of the in vivo substrate of Hsl7 and how it contributes to important cellular processes remain largely unexplored. In the present study, we show that Hsl7 has a repressive role in transcription. We found that Hsl7 is responsible for in vivo symmetric dimethylation of histone H4 arginine 3 (H4R3me2s) in a transcriptionally repressed state. Tandem affinity purification further demonstrated that Hsl7 physically interacts with histone deacetylase Rpd3, and both similarly repress transcription. Our results suggest that H4R3me2s generation by the type II PRMT Hsl7 is required for transcriptional repression, possibly in cooperation with histone deacetylation by Rpd3. (C) 2019 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S0923250819300026http://repository.hanyang.ac.kr/handle/20.500.11754/112157
ISSN
0923-2508; 1769-7123
DOI
10.1016/j.resmic.2019.01.002
Appears in Collections:
COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > MOLECULAR AND LIFE SCIENCE(분자생명과학과) > Articles
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