Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이영한 | - |
dc.date.accessioned | 2019-11-12T06:32:44Z | - |
dc.date.available | 2019-11-12T06:32:44Z | - |
dc.date.issued | 2005-12 | - |
dc.identifier.citation | EXPERIMENTAL AND MOLECULAR MEDICINE, v. 37, No. 6, Page. 601-607 | en_US |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.issn | 2092-6413 | - |
dc.identifier.uri | https://www.nature.com/articles/emm200573 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/112123 | - |
dc.description.abstract | Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Here, we report the identification of an alternatively-spliced variant of nanog. This variant lacked a stretch of amino acids (residues 168-183) located between the HD and tryptophan- repeat (WR) of the previously- reorted full length sequence, suggesting that the deleted sequence functions as a linker and possibly affects the flexibility of the C-terminal transactivation domain relative to the DNA binding domain. Expression of mRNA encoding the splice variant, designated as nanog-delta 48, was much lower than that of the full length version in human ES cells. The ratio of nanog-delta 48 transcript to full length transcript increased, however, in multipotent adult progenitor cells. EMSA analysis revealed that both forms of Nanog were able to bind a Nanog binding sequence with roughly the same affinity. A reporter plasmid assay also showed that both variants of Nanog modestly repressed transactivation of gata-4, whose expression is proposed to be inhibited by Nanog, with comparable potency. We conclude that, despite the difference in primary structure and expression pattern in various stem cells, the alternatively-spliced variant of Nanog has similar activity to that of the full length version. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | KOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY | en_US |
dc.subject | alternative splicing | en_US |
dc.subject | totipotent stem cells | en_US |
dc.subject | GATA4 transcription factor | en_US |
dc.subject | hematopoietic stem cells | en_US |
dc.subject | NANOG protein human | en_US |
dc.title | Identification and functional characterization of an alternative splice variant within the fourth exon of human nanog | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1038/emm.2005.73 | - |
dc.relation.journal | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.contributor.googleauthor | Kim, JS | - |
dc.contributor.googleauthor | Kim, J | - |
dc.contributor.googleauthor | Kim, BS | - |
dc.contributor.googleauthor | Chung, HY | - |
dc.contributor.googleauthor | Lee, YY | - |
dc.contributor.googleauthor | Park, CS | - |
dc.contributor.googleauthor | Lee, YS | - |
dc.contributor.googleauthor | Lee, YH | - |
dc.contributor.googleauthor | Chung, IY | - |
dc.relation.code | 2009210380 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF SCIENCE & TECHNOLOGY[E] | - |
dc.sector.department | DIVISION OF MOLECULAR & LIFE SCIENCE | - |
dc.identifier.pid | younghan | - |
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