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Identification and functional characterization of an alternative splice variant within the fourth exon of human nanog

Title
Identification and functional characterization of an alternative splice variant within the fourth exon of human nanog
Author
이영한
Keywords
alternative splicing; totipotent stem cells; GATA4 transcription factor; hematopoietic stem cells; NANOG protein human
Issue Date
2005-12
Publisher
KOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY
Citation
EXPERIMENTAL AND MOLECULAR MEDICINE, v. 37, No. 6, Page. 601-607
Abstract
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Here, we report the identification of an alternatively-spliced variant of nanog. This variant lacked a stretch of amino acids (residues 168-183) located between the HD and tryptophan- repeat (WR) of the previously- reorted full length sequence, suggesting that the deleted sequence functions as a linker and possibly affects the flexibility of the C-terminal transactivation domain relative to the DNA binding domain. Expression of mRNA encoding the splice variant, designated as nanog-delta 48, was much lower than that of the full length version in human ES cells. The ratio of nanog-delta 48 transcript to full length transcript increased, however, in multipotent adult progenitor cells. EMSA analysis revealed that both forms of Nanog were able to bind a Nanog binding sequence with roughly the same affinity. A reporter plasmid assay also showed that both variants of Nanog modestly repressed transactivation of gata-4, whose expression is proposed to be inhibited by Nanog, with comparable potency. We conclude that, despite the difference in primary structure and expression pattern in various stem cells, the alternatively-spliced variant of Nanog has similar activity to that of the full length version.
URI
https://www.nature.com/articles/emm200573http://repository.hanyang.ac.kr/handle/20.500.11754/112123
ISSN
1226-3613; 2092-6413
DOI
10.1038/emm.2005.73
Appears in Collections:
COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > ETC
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