Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이철훈 | - |
dc.date.accessioned | 2019-10-25T06:38:43Z | - |
dc.date.available | 2019-10-25T06:38:43Z | - |
dc.date.issued | 2006-02 | - |
dc.identifier.citation | CANCER LETTERS, v. 233, No. 1, Page. 89-97 | en_US |
dc.identifier.issn | 0304-3835 | - |
dc.identifier.issn | 1872-7980 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0304383505002235 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/111558 | - |
dc.description.abstract | In the course of screening for a novel inhibitor of cyclin-dependent kinase (CDK), HY52 (C17H30O2N2; molecular weight 294) was isolated from the leaves of Pata de Vaca (Bauhinia foificata). The growth of HeLa cells was inhibited in a dose-dependent manner when treated with 0.07 to 0.41 mM of HY52 for 24 h (IC50: 0.11 mM). Furthermore, HY52 showed the selective inhibitory activity oil CDC2 kinase purified using immunoprecipitation with all IC50 value of 0.45 mM. A flow cytometric analysis of the HeLa cells treated with HY52 revealed an,in appreciable cell-cycle arrest ill the G I phase. Moreover, a TUNEL assay exhibited the apoptotic induction of HeLa cells treated with HY52. To obtain further information on the cell-cycle arrest induced by HY52 the expression of certain cell-cycle-associated proteins was examined using a Western blot analysis. The results revealed that HY52 was found to inhibit the proliferation of HeLa cells through inducing a G1-phase arrest by inhibiting pRb phosphorylation via all up-regulation of p21(WAF1/CIPI) and p27(KIPI), and G2/M-phase arrest by down-regulation of CDC2, cyclin A, and cyclin B1. (c) 2005 Elsevier Ireland Ltd. All rights reserved. | en_US |
dc.description.sponsorship | This work was supported by research funding from Hanyang University (HY-2004-I) and by grant No. R01-2003-000-10594-0 from the Basic Research Program of the Korea Science and Engineering Foundation. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ELSEVIER SCI IRELAND LTD | en_US |
dc.subject | HY52 | en_US |
dc.subject | Bauhinia forficata | en_US |
dc.subject | Pata de Vaca | en_US |
dc.subject | cell-cycle arrest | en_US |
dc.title | Inhibition of cell-cycle progression in HeLa cells by HY52, a novel cyclin-dependent kinase inhibitor isolated from Bauhinia forficata | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1016/j.canlet.2005.03.002 | - |
dc.relation.journal | CANCER LETTERS | - |
dc.contributor.googleauthor | Lim, H | - |
dc.contributor.googleauthor | Kim, MK | - |
dc.contributor.googleauthor | Lim, Y | - |
dc.contributor.googleauthor | Cho, YH | - |
dc.contributor.googleauthor | Lee, CH | - |
dc.relation.code | 2009201684 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF PHARMACY[E] | - |
dc.sector.department | DEPARTMENT OF PHARMACY | - |
dc.identifier.pid | chhlee | - |
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