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Inhibition of cell-cycle progression in HeLa cells by HY52, a novel cyclin-dependent kinase inhibitor isolated from Bauhinia forficata

Title
Inhibition of cell-cycle progression in HeLa cells by HY52, a novel cyclin-dependent kinase inhibitor isolated from Bauhinia forficata
Author
이철훈
Keywords
HY52; Bauhinia forficata; Pata de Vaca; cell-cycle arrest
Issue Date
2006-02
Publisher
ELSEVIER SCI IRELAND LTD
Citation
CANCER LETTERS, v. 233, No. 1, Page. 89-97
Abstract
In the course of screening for a novel inhibitor of cyclin-dependent kinase (CDK), HY52 (C17H30O2N2; molecular weight 294) was isolated from the leaves of Pata de Vaca (Bauhinia foificata). The growth of HeLa cells was inhibited in a dose-dependent manner when treated with 0.07 to 0.41 mM of HY52 for 24 h (IC50: 0.11 mM). Furthermore, HY52 showed the selective inhibitory activity oil CDC2 kinase purified using immunoprecipitation with all IC50 value of 0.45 mM. A flow cytometric analysis of the HeLa cells treated with HY52 revealed an,in appreciable cell-cycle arrest ill the G I phase. Moreover, a TUNEL assay exhibited the apoptotic induction of HeLa cells treated with HY52. To obtain further information on the cell-cycle arrest induced by HY52 the expression of certain cell-cycle-associated proteins was examined using a Western blot analysis. The results revealed that HY52 was found to inhibit the proliferation of HeLa cells through inducing a G1-phase arrest by inhibiting pRb phosphorylation via all up-regulation of p21(WAF1/CIPI) and p27(KIPI), and G2/M-phase arrest by down-regulation of CDC2, cyclin A, and cyclin B1. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S0304383505002235http://repository.hanyang.ac.kr/handle/20.500.11754/111558
ISSN
0304-3835; 1872-7980
DOI
10.1016/j.canlet.2005.03.002
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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