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dc.contributor.author채영규-
dc.date.accessioned2019-10-08T01:03:27Z-
dc.date.available2019-10-08T01:03:27Z-
dc.date.issued2019-04-
dc.identifier.citationJOURNAL OF BIOLOGICAL CHEMISTRY, v. 294, NO 21, Page. 8424-8437en_US
dc.identifier.issn0021-9258-
dc.identifier.issn1083-351X-
dc.identifier.urihttp://www.jbc.org/content/294/21/8424-
dc.identifier.urihttps://repository.hanyang.ac.kr/handle/20.500.11754/110907-
dc.description.abstractMesenchymal stromal cells (MSCs) can potently regulate the functions of immune cells and are being investigated for the management of inflammatory diseases. Toll-like receptor 3 (TLR3)-stimulated human MSCs (hMSCs) exhibit increased migration and chemotaxis within and toward damaged tissues. However, the regulatory mechanisms underlying these migratory activities are unclear. Therefore, we analyzed the migration capability and gene expression profiles of TLR3-stimulated hMSCs using RNA-Seq, wound healing, and transwell cell migration assay. Along with increased cell migration, the TLR3 stimulation also increased the expression of cytokines, chemokines, and cell migration-related genes. The promoter regions of the latter showed an enrichment of putative motifs for binding the transcription factors forkhead box O1 (FOXO1), FOXO3, NF-kB (NF-kB1), and RELA proto-oncogene and NF-kB subunit. Of note, FOXO1 inhibition by the FOXO1-selective inhibitor AS1842856 significantly reduced both migration and the expression of migration-related genes. In summary, our results indicate that TLR3 stimulation induces hMSC migration through the expression of FOXO1-activated genes. © 2019 American Society for Biochemistry and Molecular Biology Inc. All rights reserved.en_US
dc.description.sponsorshipThis work was supported by National Research Foundation of Korea (NRF) Grants 2017M3A9G7073033, 2017R1A2B4012905, and 2011-0030049 (to Y. G. C.) and 2016R1D1A1B04934970 (to K. H. J.) from the Korean govern-ment. The authors declare that they have no conflicts of interest with the contents of this article.en_US
dc.language.isoenen_US
dc.publisherAMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INCen_US
dc.subjectmesenchymal stem cells (MSCs)en_US
dc.subjectToll-like receptor (TLR)en_US
dc.subjectgene expressionen_US
dc.subjectcell migrationen_US
dc.subjecttranscriptomicsen_US
dc.subjectforkhead box protein Oen_US
dc.subjectpolyinosinic:polycytidylic aciden_US
dc.subjectRNA-sequencingen_US
dc.titleForkhead box O1 (FOXO1) controls the migratory response of Toll-like receptor (TLR3)-stimulated human mesenchymal stromalcellsen_US
dc.typeArticleen_US
dc.identifier.doi10.1074/jbc.RA119.008673-
dc.relation.page1-15-
dc.relation.journalJOURNAL OF BIOLOGICAL CHEMISTRY-
dc.contributor.googleauthorKim, Sun Hwa-
dc.contributor.googleauthorDas, Amitabh-
dc.contributor.googleauthorChoi, Hae In-
dc.contributor.googleauthorKim, Ki Hoon-
dc.contributor.googleauthorChai, Jin Choul-
dc.contributor.googleauthorChoi, Mi Ran-
dc.contributor.googleauthorBinas, Bert-
dc.contributor.googleauthorPark, Kyoung Sun-
dc.contributor.googleauthorLee, Young Seek-
dc.contributor.googleauthorChai, Young Gyu-
dc.relation.code2019002362-
dc.sector.campusS-
dc.sector.daehakGRADUATE SCHOOL[S]-
dc.sector.departmentDEPARTMENT OF BIONANOTECHNOLOGY-
dc.identifier.pidygchai-
dc.identifier.orcidhttps://orcid.org/0000-0002-3333-4803-
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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