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Rapid and sensitive determination of bisphenol A using aptamer and split DNAzyme

Title
Rapid and sensitive determination of bisphenol A using aptamer and split DNAzyme
Author
계명찬
Keywords
Bisphenol A; Endocrine disruptor; Aptamer; DNAzyme; Chemiluminescence
Issue Date
2019-08
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Citation
CHEMOSPHERE, v. 228, Page. 110-116
Abstract
Despite the increasing concern regarding bisphenol A (BPA) as an endocrine disrupting chemical (EDC) upon environmental or human exposure, development of simple method for BPA detection has been hampered, due to the lack of a stable bioreceptor and signal generator. Here, we report a nucleic acid-based rapid and sensitive method for BPA detection, which constitutes a ssDNA aptamer and ssDNAzyme. When the peroxidase-like DNAzyme sequence was split into two parts (one incorporated into the anti-BPA aptamer as a target recognition element and the other into the complementary sequence as a bait), the presence of BPA hindered the association of the split DNA sequence, leading to a reduced signal in the DNAzyme-triggered chemiluminescence (CL). Thus, this NA-based CL measurement permitted the detection of BPA at as low as 5 nM with a broad dynamic range of five orders and with high selectivity towards BPA over other EDCs with structural similarity. With the development of aptamers, our detection method is expected to facilitate studies to monitor EDCs with high simplicity and sensitivity in the field of environmental science. (C) 2019 Elsevier Ltd. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S0045653519307635?via%3Dihubhttp://repository.hanyang.ac.kr/handle/20.500.11754/110781
ISSN
0045-6535; 1879-1298
DOI
10.1016/j.chemosphere.2019.04.110
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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