10 0

유산균을 이용한 경구용 항원 단백질 수송능 연구

Title
유산균을 이용한 경구용 항원 단백질 수송능 연구
Other Titles
Lactic Acid Bacteria as Oral Antigen Protein Carriers
Author
최한곤
Keywords
Lactococcous Lactis; oral carrier; protein delivery system
Issue Date
2005-04
Publisher
한국약제학회
Citation
한국약제학회지, v. 35, No. 2, Page. 75-80
Abstract
A promising application of Lactococcus lactis is its use as live vehicles for production and delivery of heterologous proteins of vaccines and therapeutic substances. Because L. lactis has GRAS ("generally regarded as safe") status, use tested whether L. lactis could function as the carrier of the L1 protein of human papillomavirus (HPV) type 16. The RNA level expression of L1 gene was detected in L. Laois. The L1 protein was expressed in L. luctis with LI gene. The growth of strains L. lactis with an empty plasmid (pAMJ328) and L. lactis with L1-encoding plasmid (pAMJ328-L1) was slightly decreased in comparison with the growth of strains L. lactis (wild type). However, all the three strains of L lactis maintained the ability to ferment sugars primarily into lactic acid, indicating that L1 protein did not affect the biochemical property of L.. lactis. These results suggest that L. lactis. capable of carrying LI protein, might he further developed as a biocompatible oral protein delivery system.
URI
http://kiss.kstudy.com/thesis/thesis-view.asp?key=2436025http://repository.hanyang.ac.kr/handle/20.500.11754/110387
ISSN
2093-5552
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE