Induction of proinflammatory mediators requires activation of the TRAF, NIK, IKK and NF‐κB signal transduction pathway in astrocytes infected with Escherichia coli
- Title
- Induction of proinflammatory mediators requires activation of the TRAF, NIK, IKK and NF‐κB signal transduction pathway in astrocytes infected with Escherichia coli
- Author
- 이철훈
- Keywords
- astrocytes; Escherichia coli; NF-kappa B; proinflammatory mediators
- Issue Date
- 2005-05
- Publisher
- BLACKWELL SCIENCE LTD
- Citation
- CLINICAL AND EXPERIMENTAL IMMUNOLOGY, v. 140, No. 3, Page. 450-460
- Abstract
- Escherichia coli is associated with inflammation in the brain. To investigate whether astrocytes are involved in E. coli-induced inflammation, we assessed the levels of expression of proinflammatory mediators produced by E. coli-infected astrocytes. E. coli infection in primary human astrocytes and cell lines increased expression of the CXC chemokine IL-8/GRO-alpha, the CC chemokine MCP-1, TNF-alpha, and iNOS. E. coli infection activated p65/p50 heterodimeric NF-kappa B and concurrently decreased the signals of I kappa B alpha. Blocking the NF-kappa B signals by I kappa B alpha-superrepressor-containing retrovirus or antisense p50 oligonucleotide transfection resulted in down-regulation of expression of the proinflammatory mediators. Furthermore, superrepressors of I kappa B alpha, I kappa B kinase (IKK) or NF-kappa B inducing kinase (NIK) inhibited the up-regulated expression of the downstream target genes of NF-kappa B such as IL-8 and MCP-1, and superrepressors of TNF receptor-associated factor (TRAF)2 and TRAF5 also inhibited expression of the E. coli-induced target genes of NF-kappa B. These results indicate that proinflammatory mediators such as the CXC chemokine IL-8/GRO-alpha, the CC chemokine MCP-1, TNF-alpha, and iNOS can be expressed in E. coli-infected astrocytes via an NF-kappa B pathway, suggesting that these mediators may contribute to inflammation in the brain, including infiltration of inflammatory cells.
- URI
- https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2249.2005.02804.xhttps://repository.hanyang.ac.kr/handle/20.500.11754/110268
- ISSN
- 0009-9104; 1365-2249
- DOI
- 10.1111/j.1365-2249.2005.02804.x
- Appears in Collections:
- COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML