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dc.contributor.author임형신-
dc.date.accessioned2019-09-04T07:28:44Z-
dc.date.available2019-09-04T07:28:44Z-
dc.date.issued2005-04-
dc.identifier.citationJOURNAL OF HEALTH SCIENCE, v. 51, No. 2, Page. 224-232en_US
dc.identifier.issn1344-9702-
dc.identifier.issn1347-5207-
dc.identifier.urihttps://www.jstage.jst.go.jp/article/jhs/51/2/51_2_224/_article/-char/ja/-
dc.identifier.urihttp://repository.hanyang.ac.kr/handle/20.500.11754/110222-
dc.description.abstractThe candidate proteins that are involved in the cyclin-dependent kinase 2 (cdk2) signaling pathway were analyzed by comparing different proteins between dominant negative cdk2 overexpressed and control HeLa cells using two-dimensional electrophoresis (2-DE) and mass spectrometry (MS). The 2-DE and MS indicated that stathmin and its monophosphorylated form were induced in etoposide-treated HeLa cells compared to untreated cells and this effect was inhibited by overexpression of dominant negative mutant form of cdk2. Further analysis showed that serine-25 (Ser-25), which comprises the conserved target motif for phosphorylation by mitogen-activated protein kinase (MAPK), was the major phosphorylation site of monophosphorylated form of stathmin. These findings indicate that etoposide-induced expression and phosphorylation at Ser-25 of stathmin might be mediated by activation of the MAPK signaling pathway, which is mediated by the cdk2 activation during the onset of the anticancer agent induced apoptotic events in the cancer cells.en_US
dc.language.isoen_USen_US
dc.publisherPHARMACEUTICAL SOC JAPANen_US
dc.subjectstathminen_US
dc.subjectcyclin-dependent kinase 2en_US
dc.subjectcancer cellen_US
dc.subjectetoposideen_US
dc.subjectproteomic analysisen_US
dc.titleAnalysis of cyclin-dependent kinase 2-regulated phosphorylation of stathmin in etoposide-induced apoptotic HeLa cells by two-dimensional electrophoresis and mass spectrometryen_US
dc.typeArticleen_US
dc.relation.volume51-
dc.identifier.doi10.1248/jhs.51.224-
dc.relation.page224-232-
dc.relation.journalJOURNAL OF HEALTH SCIENCE-
dc.contributor.googleauthorKim, Y-
dc.contributor.googleauthorKoo, KT-
dc.contributor.googleauthorChoi, JS-
dc.contributor.googleauthorJin, YH-
dc.contributor.googleauthorYim, H-
dc.contributor.googleauthorOh, YT-
dc.contributor.googleauthorLee, SK-
dc.relation.code2011205195-
dc.sector.campusE-
dc.sector.daehakCOLLEGE OF PHARMACY[E]-
dc.sector.departmentDEPARTMENT OF PHARMACY-
dc.identifier.pidhsyim-
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COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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