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NMR Studies of Zinc-binding Luteinizing Hormone Releasing Hormone

Title
NMR Studies of Zinc-binding Luteinizing Hormone Releasing Hormone
Author
원호식
Keywords
NMR; ZInc-binding LHRH
Issue Date
2006-12
Publisher
한국자기공명학회
Citation
Journal of the Korean Magnetic Resonance Society, v. 10, No. 2, Page. 163-174
Abstract
Luteinizing Hormone Releasing Hormone(LHRH) is a decapeptide neurotransmitter known to be regulated by metal ions in the hyperthalamus. Zn-binding LHRH complex was systhesized, and zinc-LHRH complex was studied to understand what kinds of structural modifications would be critical in the LHRH releasing mechanism. Both nonexchangeable and exchangeable 1H−NMR signal assignments were accomplished by pH-dependent and COSY NMR experiments. In addition, 1H−NMR chemical shift changes of a-proton and peptide NH NMR signals at different pH condition, and 1H−NMR signal differences between metal free and metallo-LHRH complex was monitored. NMR signals exhibit that primary metal-binding sites are nitrogens donor of imidazole ring and Arg, and peptide oxygen of Pro-His in the sequence. Structure obtained in this study has a cyclic conformation which is similar to that of energy minimized, and exhibits a specific a-helical turn with residue numbers (2∼7) out of 10 amino acids.
URI
http://www.koreascience.or.kr/article/JAKO200608506268063.pagehttp://repository.hanyang.ac.kr/handle/20.500.11754/109952
ISSN
1226-6531
Appears in Collections:
COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > CHEMICAL AND MOLECULAR ENGINEERING(화학분자공학과) > Articles
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