Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이민형 | - |
dc.date.accessioned | 2019-08-22T05:03:52Z | - |
dc.date.available | 2019-08-22T05:03:52Z | - |
dc.date.issued | 2019-05 | - |
dc.identifier.citation | BIOMATERIALS SCIENCE, v. 7, NO 5, Page. 2174-2190 | en_US |
dc.identifier.issn | 2047-4830 | - |
dc.identifier.issn | 2047-4849 | - |
dc.identifier.uri | https://pubs.rsc.org/en/content/articlelanding/2019/BM/C8BM01621F#!divAbstract | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/108916 | - |
dc.description.abstract | A self-assembled nanoparticle composed of hypoxia-specific anti-RAGE peptide (HSAP), heme oxygenase-1 plasmid (pHO1), and deoxycholate-conjugated polyethylenimine-2k (DP2k) was developed for ischemic stroke therapy. RAGE is over-expressed and induces inflammation in the ischemic brain. To inhibit RAGE-mediated signal transduction, HSAP was produced by recombinant DNA technology, based on the RAGE-binding domain of high mobility group box-1. Because of the specific binding to RAGE, the nanoparticle with HSAP (HSAP-NP) may have dual roles as a cytoprotective reagent and a specific ligand to RAGE for receptor-mediated transfection. As a cytoprotective reagent, the HSAP-NP reduced RAGE expression on the surface of the brain cells by inhibiting the positive feedback of RAGE-mediated signal transduction. As a result, inflammation, apoptosis, and reactive oxygen species were decreased in hypoxic cells. As a gene carrier, HSAP-NP showed a higher transfection efficiency than polyethylenimine-25k, DP2k, and Lipofectamine. Particularly, HSAP-NP enhanced gene delivery to hypoxic cells. In the stroke animal models, HSAP-NP reduced the levels of RAGE, inducible nitric oxide synthase, and inflammation. Additionally, HSAP-NP with pHO1 (HSAP-NP/pHO1) increased HO1 expression in the ischemic brain. Gene expression was higher in hypoxia-inducible factor-1 (HIF-1)-positive cells than in HIF-1-negative cells, suggesting that HSAP-NP delivered the genes to ischemic tissues more efficiently. Cell death and infarct volume in the stroke models were significantly decreased by HSAP-NP/pHO1 compared with HSAP alone or the DP2k/pHO1 complex. Therefore, HSAP-NP may be a useful gene and peptide therapy system for stroke therapy with dual functions of hypoxia-specific gene delivery and cytoprotective effects. | en_US |
dc.description.sponsorship | This work was supported by the Individual Basic Science & Engineering Research Program (NRF-2017R1A2B4009036) and Bio & Medical Technology Development Program (NRF-2016M3A9B4918833) through the National Research Foundation funded by the Ministry of Science and ICT. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ROYAL SOC CHEMISTRY | en_US |
dc.subject | HEME OXYGENASE-1 GENE | en_US |
dc.subject | RAGE-BINDING PEPTIDE | en_US |
dc.subject | CONJUGATED POLYETHYLENIMINE | en_US |
dc.subject | KAPPA-B | en_US |
dc.subject | RECEPTOR | en_US |
dc.subject | DELIVERY | en_US |
dc.subject | EXPRESSION | en_US |
dc.subject | CELLS | en_US |
dc.subject | HMGB1 | en_US |
dc.subject | NEUROINFLAMMATION | en_US |
dc.title | A self-assembled DNA-nanoparticle with a targeting peptide for hypoxia-inducible gene therapy of ischemic stroke | en_US |
dc.type | Article | en_US |
dc.relation.no | 5 | - |
dc.relation.volume | 7 | - |
dc.identifier.doi | 10.1039/c8bm01621f | - |
dc.relation.page | 2174-2190 | - |
dc.relation.journal | BIOMATERIALS SCIENCE | - |
dc.contributor.googleauthor | Oh, Jungju | - |
dc.contributor.googleauthor | Lee, Jaewon | - |
dc.contributor.googleauthor | Piao, Chunxian | - |
dc.contributor.googleauthor | Jeong, Ji Hoon | - |
dc.contributor.googleauthor | Lee, Minhyung | - |
dc.relation.code | 2019039036 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | minhyung | - |
dc.identifier.orcid | http://orcid.org/0000-0002-7083-9296 | - |
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