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Development of an open sandwich fluoroimmunoassay based on fluorescence resonance energy transfer

Title
Development of an open sandwich fluoroimmunoassay based on fluorescence resonance energy transfer
Author
조용우
Keywords
FRET; Sandwich assay; Quantum dots; Fluorescence labels; Sandwich immunoassays
Issue Date
2006-11
Publisher
ACADEMIC PRESS INC
Citation
ANALYTICAL BIOCHEMISTRY, v. 358, No. 1, Page. 31-37
Abstract
We have developed a sensitive, one-step, homogeneous open sandwich fluoroimmunoassay (OsFIA) based on fluorescence resonance energy transfer (FRET) and luminescent semiconductor quantum dots (QDs). In this FRET assay, estrogen receptor beta (ER-beta) antigen was incubated with QD-labeled anti-ER-beta monoclonal antibody and Alexa Fluor (AF)-labeled anti-ER polyclonal antibody for 30 min, followed by FRET measurement. The dye separation distance was estimated between 80 and 90 angstrom. The current method is rapid, simple, and highly sensitive, and it did not require the bound/free reagent separation steps and solid-phase carriers. A concentration as low as 0.05 nM (2.65 ng/ml) receptor was detected with linearity. In addition, the assay was performed with commercial antibodies. This assay provides a convenient alternative to conventional, laborious sandwich immunoassays. (c) 2006 Elsevier Inc. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S000326970600604Xhttp://repository.hanyang.ac.kr/handle/20.500.11754/108877
ISSN
0003-2697
DOI
10.1016/j.ab.2006.08.019
Appears in Collections:
COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > MATERIALS SCIENCE AND CHEMICAL ENGINEERING(재료화학공학과) > Articles
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